Sodium Potassium ATPase (ATP1A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	WB 1:500 - 1:1000;IP 1:20 - 1:50
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein of human ATP1A1
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	113 kDa
Gene Name	ATPase Na+/K+ transporting subunit alpha 1
Database Link	NP_000692 Entrez Gene 476 Human UniProt ID P05023
Background	The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic a subunit and a β subunit . Several phosphorylation sites have been identified for the a1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase , Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA . All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule . Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the a subunit . Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors.
Synonyms	MGC3285; MGC51750
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cardiac muscle contraction

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