Caspase 3 (CASP3) Mouse Monoclonal Antibody [Clone ID: 31A1067]

CAT#: TA336455

Mouse Monoclonal Caspase-3 (Pro and Active) Antibody (31A1067)



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CNY 6,145.00


货期*
5周

规格
    • 100 ug

经常一起买 (5)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

CNY 300.00
CNY 1,430.00


Recombinant protein of human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, 20 µg
    • 20 ug

CNY 2,900.00


Transient overexpression lysate of caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha
    • 100 ug

CNY 3,080.00


Recombinant protein of human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, 100 µg
    • 100 ug

CNY 9,998.00


Transient overexpression lysate of caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
    • 100 ug

CNY 3,080.00

Specifications

Product Data
Clone Name 31A1067
Applications CyTOF-ready, Electron Microscopy, FC, ICC/IF, IHC, Immunoblotting, Simple Western, WB
Recommend Dilution Immunohistochemistry: 1:10 - 1:500, Simple Western: 1:50, Western Blot: 1 - 5 ug/ml, Knockout Validated, Knockdown Validated, Immunohistochemistry-Frozen: 1:10 - 1:500, Immunohistochemistry-Paraffin: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence, Flow Cytometry, Electron Microscopy, Immunoblotting, Hematoxylin and Eosin Stain, Immunohistochemistry Free-Floating, CyTOF-ready
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
Formulation PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Gene Name caspase 3
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
Synonyms CPP32; CPP32B; SCA-1
Note Useful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553).
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protease
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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