Insulin (INS) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against C- Peptide. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Glu-Ala-Glu-Asp-Leu-Gln-Val-Gly-Gln-Val-Glu- Leu- Gly-Gly-Gly-Pro-Gly-Ala-Gly-Ser-Leu-Gln-Pro-Leu-Ala-Leu- Glu-Gly-Ser- Leu-Gln-OH coupled to carrier protein.
Formulation	Each vial contains enough antiserum for 500 RIA tubes. The powder should be rehydrated with 50ml RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	insulin
Database Link	Entrez Gene 3630 Human UniProt ID P01308
Background	The connecting peptide, or C-peptide of human proinsulin, is a 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain. In the context of diabetes or hypoglycemia, a measurement of C-peptide blood serum levels can be used to distinguish between different conditions with similar clinical features. In normal tissues, the antigen is found in pancreaticislet β-cells. C-peptide has been found to be a bioactive peptide in its own right, with effectson microvascular blood flow and tissue health. This antibody was generated by immunization of rabbits with coupled to a carrier protein.
Synonyms	ILPR; insulin; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.