Edn1 Rabbit Polyclonal Antibody

CAT#: TA364259

Rabbit polyclonal anti Big Endothelin-1 (rt); diluted antiserum



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CNY 11,025.00


货期*
5周

规格
    • 500 Tests

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Specifications

Product Data
Applications ELISA
Recommend Dilution This antibody has been tested and validated in ELISA against Big Endothelin-1. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys- Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val- Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser-OH (Disulfide bridge: 1-15 and 3-11) coupled to carrier protein.
Formulation Each vial contains enough antiserum for 500 RIA tubes. The powder should be rehydrated with 50ml RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4°C. This should ensure antibody stability for approximately one month.
Concentration N/A
Conjugation Unconjugated
Storage Condition Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name endothelin 1
Background Big Endothelin-1 (1-38) is a biological precursor of endothelin 1 (1-21). It is cleaved to yield endothelin-1 via the activity of an endothelin-converting enzyme (ECE), a membrane-bound metalloproteinase, that leads to the active Endothelin (1-21) and to the biological inactive C- terminal fragment (22-38). Big Endothelin-1 can be hydrolyzed by chymase to generate endothelin 1 (1-21) in vitro. This antibody was generated by immunization of rabbits with Big Endothelin-1 coupled to a carrier protein.
Synonyms ET1; HDLCQ7; PPET1; Preproendothelin-1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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