Tnf Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500 - 1:2,000 IHC: 1:100 - 1:500 ELISA: 1:1,000 - 1:5,000
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.
Specificity	This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin).
Formulation	None
Concentration	85 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 21926 Mouse UniProt ID P06804
Background	TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.
Synonyms	rabbit anti-Mouse TNF alpha Antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Note	This antiserum against Mouse TNFa has been tested for use in immunoblotting.  This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, immunoprecipitation, and immunohistochemistry. Reactivity in other immunoassays is unknown. It recognizes the 17,000 MW TNFa. This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of mouse activity in bioassays.  It does not neutralize the biological activity of lymphotoxin.  For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.
Reference Data

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