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Mia2 (NM_146034) Mouse Tagged ORF Clone
CAT＃: MG210606
- TrueORF®
Ctage5 (tGFP-tagged) - Mouse meningioma expressed antigen 6 (coiled-coil proline-rich) (Mgea6)
ORF Plasmid: DDK
"NM_146034" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210606 representing NM_146034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGCAGACTTTAATCCCAGTGGTTTTTCGTTGGAATTGGCAGTATGTGTGCTTTCCGTTGGACTGC TTGCCGTGGTCTTGTTTCTGTGGCGAGGCTTCCGATCAATTCGAAGCCGATTTTATGTGGGGAGAGAAAA GAAACTTGCTCTTGAGCTTTCTGCACTAATTGAAGAAAAATGTAAACTGCTTGACAAAGTCAGCATTGTT CAAAAGGAGTATGAAGGCTTAGAGTCATCTTTAAAGGAGGCCAGCTTTGAGAAGGAGTCAACAGAAGCAC AAAGTTTGGAGTTTGTTGAAGGATCACAAATATCAGAGGCCACCTATGAAAATCTGGAACAATCCAAATC TAAACTTGAAGATGAAATACTACTCCTAGAGGAAAAGCTGGAAGAAGAGCGAGCTAAACATTCTGAACAA GACGAGCTGATGGCTGATATTTCAAAAAGGATACAGTCCCTAGAAGATGAATCAAAATCTCTCAAATCGC AAGTAGCTGAAGCCAAAACAACCTTTAGAATATTTGAAATCAATGAAGAGCGACTCAAGGGAGCAATAAA AGATGCCTTGAATGAAAATTCTCAGCTTCAGGAAAGCCAGAAACAGCTTTTACAGGAAACTGAAATGATG AAAGAACAAGTGAATGATCTCGATAAACAGAAAGTGGCATTGGAAGAGTCCAGAGCACAGGCGGAACAAG CTCTGAGTGAGAAGGAGAGTCAGATTGAGACTCTGGTCACGAGCCTGCTAAAGATGAAGGATTGGGCTGC TGTGCTTGGAGAAGACATAGCAGATGATGGGAATCTGGACTTGGACATGAAGAGTGGCCTAGAAAACACT GCTGCCTTAGATAATCAGCCAAAGGGCGCTTTGAAGAAGCTGATTTATGCAGCTAAGTTAAATGCTTCTT TAAAAGCCTTGGAAGGAGAACGAAATCAAGTTTACACTCAGTTATCTGAAGTGGATCAAGTAAAAGAAGA CCTTACAGAGCATATCAAAAGTCTTGAGTCTAAACAAGCATCTTTGCAGTCAGAAAAGACAGAGTTTGAA AGTGAGAGCCAGAAACTTCAGCAGAAACTGAAAGTGATAACCGAGCTGTACCAAGAAAATGAAATGAAAC TTCACAGGAAATTAACAGTAGAAGAAAATTACCGATTAGAGAAAGAAGAAAAACTTTCCAAAGTAGATGA GAAAATCAGCCATGCGACCGAGGAGCTGGAGACCTGCAGGCAGCGTGCCAAGGATCTTGAAGAAGAGCTG GAGAGAACAATTCATTCTTACCAGGGGCAGGTTATTTCTCATGAAAAAAAAGCACATGATAATTGGCTGG CAGCTCGGACTCTTGAAAGAAATCTCAATGATTTAAGAAAAGAAAATGCCCACAACAGACAAAAATTAAC TGAAACAGAGTTTAAATTTGAACTTTTAGAAAAAGATCCTTATGCACTTGATGTTCCAAACACAGCATTT GGCAGAGAGCACTCCCCATATGGTCCCTCACCATTGGGCCGACCTCCATCTGAAACGAGAGCTTTTCTCT CTCCTCCAACTTTGTTGGAGGGTCCACTAAGACTCTCACCTTTGCTTCCAGGGGGAGGAGGAAGAGGCTC ACGAGGCCCTGAGAACCTTCTGGACCATCAGATGAACACTGAAAGAGGAGAGTCAAGCTATGACAGATTA TCTGATGCTCCCAGAGCACCTTCCGACAGGTCCCTGTCACCTCCGTGGGAACAAGATCGAAGGATGACAG CCCATCCGCCACCAGGCCAACCATATTCTGATCCAGCTCTACAAAGGCAAGATCGGTTTTATCCTAATTC TGGTAGACTCTCTGGACCAGCAGAACTCAGAAGTTATAATATGCCCTCTTTGGATAAAGTGGATGGGCCA GTACCCTCAGAAATGGAGTCCAGTGGAAACGGTACCAAAGATAATCTTGGTAACTCTAATGTGCCTGATT CTCCTATCCCTGCTGAATGCGAAGCAGCTGGCCGTGGCTTCTTCCCTCCACCCTTTCCTCCAGTCAGAGA CCCATTGTTTCCAGTGGATCCAAGGAGCCAGTTCATGAGAAGGGGCCCCTCTTTCCCCCCACCTCCTCCA GGAAGCATCTATGCAGCTCCTCGAGATTATTTTCCACCAAGGGACTTCCCTGGCCCACCACTTCCTCCGT TCCCAGGGAGAACTGTGTACGCACCGAGGGGCTTTCCACCTTACCTTCCCCCCAGAGCTGGGTTCTTCCC CCCACCCCCACATCCTGAAAGTAGAAGTGAGCTCCCCCCAGACTTGATTCCGCCTTCAAAAGAGCCTGCA GCTGACCCTCCAGAAACACAGGAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210606 representing NM_146034
Red=Cloning site Green=Tags(s) MRADFNPSGFSLELAVCVLSVGLLAVVLFLWRGFRSIRSRFYVGREKKLALELSALIEEKCKLLDKVSIV QKEYEGLESSLKEASFEKESTEAQSLEFVEGSQISEATYENLEQSKSKLEDEILLLEEKLEEERAKHSEQ DELMADISKRIQSLEDESKSLKSQVAEAKTTFRIFEINEERLKGAIKDALNENSQLQESQKQLLQETEMM KEQVNDLDKQKVALEESRAQAEQALSEKESQIETLVTSLLKMKDWAAVLGEDIADDGNLDLDMKSGLENT AALDNQPKGALKKLIYAAKLNASLKALEGERNQVYTQLSEVDQVKEDLTEHIKSLESKQASLQSEKTEFE SESQKLQQKLKVITELYQENEMKLHRKLTVEENYRLEKEEKLSKVDEKISHATEELETCRQRAKDLEEEL ERTIHSYQGQVISHEKKAHDNWLAARTLERNLNDLRKENAHNRQKLTETEFKFELLEKDPYALDVPNTAF GREHSPYGPSPLGRPPSETRAFLSPPTLLEGPLRLSPLLPGGGGRGSRGPENLLDHQMNTERGESSYDRL SDAPRAPSDRSLSPPWEQDRRMTAHPPPGQPYSDPALQRQDRFYPNSGRLSGPAELRSYNMPSLDKVDGP VPSEMESSGNGTKDNLGNSNVPDSPIPAECEAAGRGFFPPPFPPVRDPLFPVDPRSQFMRRGPSFPPPPP GSIYAAPRDYFPPRDFPGPPLPPFPGRTVYAPRGFPPYLPPRAGFFPPPPHPESRSELPPDLIPPSKEPA ADPPETQEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146034 |
| ORF Size | 2331 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146034.1 |
| RefSeq Size | 2838 bp |
| RefSeq ORF | 2385 bp |
| Locus ID | 338320 |
| Gene Summary | This gene encodes a protein that is involved in endoplasmic reticulum-to-Golgi trafficking and regulation of cholesterol metabolism. Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species. Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding diverse isoforms. A mutation in this gene (couch-potato or cpto) may result in low levels of plasma cholesterol and triglycerides. [provided by RefSeq, Sep 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205435 | Ctage5 (untagged) - Mouse CTAGE family, member 5 (Ctage5), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MR210606 | Ctage5 (Myc-DDK-tagged) - Mouse CTAGE family, member 5 (Ctage5), transcript variant 1 |
CNY 5,712.00 |
|
| MR210606L3 | Lenti ORF clone of Ctage5 (Myc-DDK-tagged) - Mouse CTAGE family, member 5 (Ctage5), transcript variant 1 |
CNY 7,700.00 |
|
| MR210606L4 | Lenti ORF clone of Ctage5 (mGFP-tagged) - Mouse CTAGE family, member 5 (Ctage5), transcript variant 1 |
CNY 7,700.00 |
