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Gtf3a (NM_025652) Mouse Tagged ORF Clone
CAT＃: MG223388
- TrueORF®
Gtf3a (tGFP-tagged) - Mouse general transcription factor III A (Gtf3a), (10ug)
ORF Plasmid: DDK
"NM_025652" in other vectors (4)
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CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2010015D03Rik; 2610111I01Rik; 5330403M05Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223388 representing NM_025652
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGAGCCGCGGGTGTCAGTCGCGGAAGCGGTGTCGTCGCTGACCATCGCGGATGCGTTCGTCGGGGCCT GTGAGGGCCCCGCGCCGCCGCGCCCCGCGCTGCCCAGCAGGTTCATCTGCTCCTTTCCCGACTGCAGCGC CAGTTACAACAAAGCCTGGAAGCTAGACGCGCACCTATGCAAACACACGGGGGAGAGGCCATTTGTTTGC GACTATGAGGGCTGTGGCAAGGCCTTCATCAGAGACTACCATCTGAGCCGGCATGTCCTGATTCACACCG GGGAAAAGCCGTTTGTTTGTGCAGATGATGGCTGTAATCAGAAATTCAACACAAAATCAAACTTGAAGAA ACACATTGAACGCAAACATGGAAACCCACAAAAACAGTATGTGTGCAGTTATGAGGGTTGCAAGAAGGCC TTTAAGAAGCACCAGCAGCTGAGAACCCATCAGTGCCAGCACACCAGCGAGCCGCTCTTCAGGTGTACCC ACGAGGGATGCGGGAAGCACTTTGCCTCGCCCAGCAGGCTGAAACGGCATGGGAAAGTTCACGAGGGCTA CCTGTGTCAAAAGGGATGTTCTTTCATGGGAAAAACGTGGACAGAGCTCCTGAAACACATGAGAGAAGCC CATAAAGAGGACATAACCTGCAATGTATGTCAGAGGATGTTCAAGCGCAGAGATTACCTTAAGCAGCACA TGAAGACTCACGCCCCGGAAAGGGATGTGTACCGCTGTCCGCGGCAAGGCTGCGGAAGAACCTACACAAC CGTGTTCAACCTGCAGAGCCACATTCTCTCCTTCCACGAGGAAAAGCGCCCATTTGTGTGTGAGCACGCT GGCTGTGGCAAGACATTCGCAATGAAACAGAGTCTCATGAGGCACAGTGTCGTGCACGATCCCGACAAGA AGAGGATGAAGCTCAAAGTAAGAGCCCCTCGGGAGAGACGCAGCTTGGCCTCTCGCCTCAGTGGGTACTT CCCTCCTAAGAGGAAACAAGAGCCCGACTACTCCTTGCCTAACGCCAGCGCAGAGTCCAGCAGCAGCCCA GAGGCCCAGCTGCCCCCGCCAGCCGCCTTACTCACTGTCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223388 representing NM_025652
Red=Cloning site Green=Tags(s) LEPRVSVAEAVSSLTIADAFVGACEGPAPPRPALPSRFICSFPDCSASYNKAWKLDAHLCKHTGERPFVC DYEGCGKAFIRDYHLSRHVLIHTGEKPFVCADDGCNQKFNTKSNLKKHIERKHGNPQKQYVCSYEGCKKA FKKHQQLRTHQCQHTSEPLFRCTHEGCGKHFASPSRLKRHGKVHEGYLCQKGCSFMGKTWTELLKHMREA HKEDITCNVCQRMFKRRDYLKQHMKTHAPERDVYRCPRQGCGRTYTTVFNLQSHILSFHEEKRPFVCEHA GCGKTFAMKQSLMRHSVVHDPDKKRMKLKVRAPRERRSLASRLSGYFPPKRKQEPDYSLPNASAESSSSP EAQLPPPAALLTVC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025652 |
| ORF Size | 1092 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025652.3, NP_079928.2 |
| RefSeq Size | 1315 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 66596 |
| UniProt ID | Q8VHT7 |
| Gene Summary | The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204444 | Gtf3a (untagged) - Mouse general transcription factor III A (Gtf3a), (10ug) |
CNY 2,000.00 |
|
| MR223388 | Gtf3a (Myc-DDK-tagged) - Mouse general transcription factor III A (Gtf3a) |
CNY 3,610.00 |
|
| MR223388L3 | Lenti ORF clone of Gtf3a (Myc-DDK-tagged) - Mouse general transcription factor III A (Gtf3a) |
CNY 5,510.00 |
|
| MR223388L4 | Lenti ORF clone of Gtf3a (mGFP-tagged) - Mouse general transcription factor III A (Gtf3a) |
CNY 5,510.00 |
