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Hsd11b2 (NM_008289) Mouse Tagged ORF Clone
CAT＃: MR206216
- TrueORF®
Hsd11b2 (Myc-DDK-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 2 (Hsd11b2)
ORF Plasmid: tGFP
"NM_008289" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 11HSD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206216 representing NM_008289
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCTGGCCTTGGCCGTCGGGCGGCGCCTGGCTGCTGGTGGCTGCCCGCGCGCTGCTGCAGCTGC TGCGCTCAGACCTGCGTCTGGGCCGCCCGTTGTTGGCGGCACTGGCCCTGCTGGCTGCTCTCGACTGGCT GTGCCAGCGCCTGCTGCCCCCGCCGGCGGCACTCGTGGTGCTGGCTGGTGCTGGCTGGATCGCGTTGTCC CGCCTAGCGCGCCCTCCTCGCCTGCCGGTGGCCACTCGCGCGGTGCTCATCACCGGTTGTGACACTGGTT TTGGCAAGGAGACAGCTAAGAAACTGGATGCCATGGGCTTCACGGTGCTGGCCACAGTGTTGGATTTGAA TAGCCCTGGTGCCCTAGAACTGCGTGACCTCTGTTCTCCTCGCCTGAAGCTGCTGCAGATGGATCTGACC AAGGCAGAGGACATCAGCCGTGTTCTGGAAATCACCAAGGCCCACACGGCCAGCACTGGCCTGTGGGGTC TGGTTAACAACGCTGGCCTCAATATCGTAGTGGCTGACGTGGAACTGTCTCCAGTGGCGACTTTCCGCAA GTGCATGGAGGTGAACTTCTTTGGTGCACTTGAGCTGACCAAGGGCCTCCTGCCACTCTTGCGTCACTCG AGGGGACGTATTGTGACCGTTGGCAGCCCAGCAGGAGACATGCCATACCCCTGCTTGGCAGCCTACGGCA CCTCCAAGGCAGCAATAGCACTGCTTATGGACACATTCGGCTGTGAGCTGCTTCCCTGGGGTATCAAGGT CAGCATTATCAAGCCTGGCTGCTTCAAGACAGATGCAGTGACTAATGTGAACCTCTGGGAGAAACGCAAG CAACTGCTGCTGGCCAACATTCCTAGAGAGCTGCTCCAGGCCTATGGTGAAGACTACATTGAGCACGTGC ACGGGCAGTTCCTGAATTCACTCAGAATGGCATTGCCTGACCTTAGCCCAGTTGTAGATGCCATCATCGA TGCATTGCTGGCAGCTCAGCCACGAAGCCGCTACTACCCAGGCCGTGGCCTGGGGCTCATGTATTTCATC CACCACTACCTGCCAGAGGGCCTGCGACGCTGCTTCCTACAGAACTTCTTTATCAATCACCTTCTGCCCC GAGCACTGAGGCCCGGCCAACATGGCCCTGCTCCTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008289 |
| ORF Size | 1158 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008289.2, NP_032315.2 |
| RefSeq Size | 1874 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 15484 |
| UniProt ID | P51661 |
| MW | 42.6 kDa |
| Gene Summary | Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202925 | Hsd11b2 (untagged) - Mouse hydroxysteroid 11-beta dehydrogenase 2 (Hsd11b2), (10ug) |
CNY 2,000.00 |
|
| MG206216 | Hsd11b2 (tGFP-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 2 (Hsd11b2) |
CNY 3,710.00 |
|
| MR206216L3 | Lenti ORF clone of Hsd11b2 (Myc-DDK-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 2 (Hsd11b2) |
CNY 5,230.00 |
|
| MR206216L4 | Lenti ORF clone of Hsd11b2 (mGFP-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 2 (Hsd11b2) |
CNY 5,230.00 |
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