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Acadm (NM_007382) Mouse Tagged ORF Clone
CAT＃: MR206704
- TrueORF®
Acadm (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_007382" in other vectors (3)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU018656; MCA; MCAD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206704 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGCGTTCCGCAGAGGCTGCAGGGTCCTGAGAAGTGTTTCTCATTTTGAGTGTCGAACACAAC ACTCGAAAGCGGCTCACAAGCAGGAGCCCGGATTAGGGTTTAGTTTTGAGTTGACGGAACAGCAGAAAGA GTTTCAAGCAACTGCCCGCAAGTTTGCCAGAGAGGAGATTATCCCCGTCGCCCCGGAATATGACAAAAGC GGGGAGTACCCGTTCCCTCTCATCAAAAGAGCCTGGGAACTCGGCTTGATCAACGCGCACATTCCGGAAA GTTGCGGTGGTCTTGGCCTGGGAACGTTCGATGCTTGTTTAATTACCGAAGAGTTGGCGTATGGGTGTAC AGGGGTGCAAACTGCTATTGAAGCAAATTCTTTGGGGCAAATGCCTGTGATTCTTGCTGGAAATGATCAA CAAAAAAAGAAGTATTTGGGGAGGATGACGGAGCAGCCAATGATGTGTGCTTACTGTGTGACAGAGCCCT CCGCAGGCTCTGATGTGGCGGCCATTAAGACCAAAGCAGAGAAGAAGGGTGACGAGTATGTTATCAACGG CCAGAAGATGTGGATAACCAACGGGGGAAAGGCCAACTGGTATTTCTTGTTGGCGCGTTCTAACCCAGAT CCTAAAGTACCCGCTAGTAAAGCCTTTACTGGATTCATTGTGGAAGCCGACACCCCGGGAATACATATTG GAAAAAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGT GCCTAAGGAAAATGTGTTAATCGGTGAAGGAGCAGGTTTCAAGATCGCAATGGGTGCTTTTGATAGAACC AGACCTACAGTCGCAGCTGGCGCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCC TGGATAGGAAGACATTTGGAAAGCTGCTAGTGGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGC GATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAGCCTGGGAGGTTGACTCCGGTCGCCGGAAC ACTTACTATGCCTCGATTGCAAAGGCCTGTGCTGGAGACATTGCCAATCAGCTAGCCACTGACGCCGTGC AGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGCCAAGATCTA TCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAA AAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206704 protein sequence
Red=Cloning site Green=Tags(s) MAAAFRRGCRVLRSVSHFECRTQHSKAAHKQEPGLGFSFELTEQQKEFQATARKFAREEIIPVAPEYDKS GEYPFPLIKRAWELGLINAHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVILAGNDQ QKKKYLGRMTEQPMMCAYCVTEPSAGSDVAAIKTKAEKKGDEYVINGQKMWITNGGKANWYFLLARSNPD PKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGIAFEDVRVPKENVLIGEGAGFKIAMGAFDRT RPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRN TYYASIAKACAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYK N TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007382 |
| ORF Size | 1266 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007382.1, NM_007382.2, NM_007382.3, NM_007382.4, NM_007382.5, NP_031408.1 |
| RefSeq Size | 2062 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 11364 |
| UniProt ID | P45952 |
| MW | 46.4 kDa |
| Gene Summary | This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206704 | Acadm (tGFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm) |
CNY 3,140.00 |
|
| MR206704L3 | Lenti ORF clone of Acadm (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR206704L4 | Lenti ORF clone of Acadm (mGFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
