Asgr1 (NM_001291131) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ASGPR1; Asgr; Asgr-1; HL-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228994 representing NM_001291131 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAAGGATTATCAAGATTTCCAGCACCTGGACAATGATAATGACCATCATCAACTCCGGAGAGGGC CGCCTCCCACTCCACGGCTCTTGCAGCGACTCTGCTCTGGATCCCGCCTCCTCCTGCTCTCCTCGAGCCT CAGCATTCTGTTGCTGGTGGTTGTCTGTGTGATCACATCCCAAAATTCCCAACTCCGGGAAGATCTGCTG GCTCTAAGGCAGAATTTCAGCAACCTCACTGTGAGCACTGAGGACCAGGTCAAGGCCCTGAGCACCCAGG GAAGTAGTGTGGGAAGAAAGATGAAGTTAGTGGAGTCGAAGCTGGAAAAACAGCAGAAGGATCTGACTGA AGATCACTCCAGTTTGCTACTGCACGTGAAGCAGTTAGTGTCTGACGTGCGAAGCTTGAGCTGCCAGATG GCTGCATTTCGGGGCAATGGCTCTGAAAGGACCTGCTGCCCCATCAACTGGGTGGAGTATGAAGGCAGCT GCTACTGGTTCTCCAGCTCTGTGAGGCCTTGGACTGAAGCTGACAAGTACTGCCAGCTGGAAAATGCCCA TCTGGTGGTGGTGACCTCCAGGGATGAGCAGAACTTCCTCCAGCGCCACATGGGCCCCTTAAACACTTGG ATTGGCCTAACTGACCAGAACGGGCCCTGGAAATGGGTGGATGGAACAGACTACGAGACAGGCTTCCAGA ATTGGAGACCAGAGCAGCCAGATAACTGGTACGGACATGGGCTTGGAGGAGGCGAGGACTGTGCCCACTT CACGACGGATGGCCGCTGGAATGACGACGTCTGCAGGAGGCCCTACCGCTGGGTCTGTGAGACAAAGTTG GATAAGGCTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228994 representing NM_001291131 Red=Cloning site Green=Tags(s) MTKDYQDFQHLDNDNDHHQLRRGPPPTPRLLQRLCSGSRLLLLSSSLSILLLVVVCVITSQNSQLREDLL ALRQNFSNLTVSTEDQVKALSTQGSSVGRKMKLVESKLEKQQKDLTEDHSSLLLHVKQLVSDVRSLSCQM AAFRGNGSERTCCPINWVEYEGSCYWFSSSVRPWTEADKYCQLENAHLVVVTSRDEQNFLQRHMGPLNTW IGLTDQNGPWKWVDGTDYETGFQNWRPEQPDNWYGHGLGGGEDCAHFTTDGRWNDDVCRRPYRWVCETKL DKAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291131
ORF Size	852 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291131.1, NP_001278060.1
RefSeq Size	1150 bp
RefSeq ORF	855 bp
Locus ID	11889
UniProt ID	P34927
MW	32.6 kDa
Gene Summary	Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.[UniProtKB/Swiss-Prot Function]

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