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Flavin containing monooxygenase 4 (FMO4) (NM_002022) Human Tagged ORF Clone
CAT＃: RC201181
FMO4 (Myc-DDK-tagged)-Human flavin containing monooxygenase 4 (FMO4)
ORF Plasmid: tGFP
"NM_002022" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FMO2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201181 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGAAAGTTGCAGTGATTGGAGCTGGTGTGAGTGGCCTCTCCTCCATCAAATGCTGTGTGGATG AGGACCTGGAGCCCACCTGCTTTGAGAGAAGTGATGACATTGGGGGATTATGGAAGTTTACTGAATCTTC CAAAGATGGGATGACCAGGGTCTATAAGTCATTAGTGACAAATGTCTGTAAGGAAATGTCATGTTACAGT GACTTCCCTTTCCACGAAGATTATCCTAATTTCATGAACCATGAAAAATTTTGGGACTATCTCCAAGAAT TTGCTGAGCACTTTGACCTCCTGAAATACATTCAGTTTAAGACCACTGTGTGCAGCATAACGAAGCGTCC AGACTTCTCCGAAACTGGTCAGTGGGATGTTGTCACAGAGACAGAGGGCAAGCAAAATAGAGCTGTCTTT GATGCTGTTATGGTTTGCACTGGACATTTCCTGAATCCCCATTTACCTTTGGAAGCCTTTCCTGGAATTC ATAAGTTTAAAGGTCAGATCCTGCATAGTCAAGAGTACAAGATCCCAGAAGGCTTTCAGGGCAAACGCGT CTTGGTGATTGGTCTTGGGAACACTGGAGGAGACATTGCTGTGGAACTCAGTCGAACGGCAGCTCAGGTA CTTCTCAGTACTAGAACTGGTACCTGGGTTCTTGGGCGCTCTTCAGATTGGGGCTATCCTTATAATATGA TGGTTACAAGAAGATGCTGTAGTTTTATTGCACAAGTTCTGCCTTCACGTTTTCTAAACTGGATTCAAGA AAGGAAGTTGAATAAGAGATTTAATCATGAGGATTATGGATTAAGTATTACCAAAGGGAAAAAAGCAAAA TTCATTGTGAATGATGAGCTGCCAAACTGTATCCTCTGTGGGGCAATCACTATGAAAACCAGCGTGATTG AATTTACAGAAACCTCTGCTGTCTTTGAAGATGGGACAGTGGAAGAAAACATTGATGTTGTGATCTTCAC TACAGGATATACATTTTCTTTTCCATTTTTTGAAGAACCTCTTAAAAGCCTCTGTACAAAGAAGATATTT CTATACAAGCAAGTCTTTCCCTTAAACCTAGAGAGAGCGACATTAGCCATCATCGGCCTTATCGGCCTTA AAGGATCCATCTTATCAGGCACAGAGCTCCAAGCACGATGGGTCACAAGAGTATTCAAAGGACTCTGTAA GATACCTCCATCCCAAAAATTGATGATGGAGGCTACTGAAAAGGAACAGCTCATTAAAAGGGGAGTGTTT AAAGACACCAGCAAAGACAAATTTGACTACATTGCCTACATGGATGATATCGCTGCCTGCATAGGCACAA AGCCCAGCATCCCACTTCTGTTCCTCAAGGATCCCAGACTAGCTTGGGAAGTTTTCTTTGGACCATGTAC TCCTTATCAGTACCGCCTCATGGGCCCTGGAAAATGGGATGGAGCCAGAAATGCCATCCTGACCCAGTGG GACAGAACATTGAAACCTTTAAAAACTCGAATTGTCCCTGATTCCTCCAAGCCTGCCTCCATGTCACATT ATTTAAAAGCCTGGGGGGCACCTGTCCTACTTGCCTCTCTTCTACTTATCTGTAAATCTTCACTTTTCTT GAAATTGGTGAGAGATAAACTACAGGACAGAATGTCCCCTTACCTAGTAAGTCTTTGGCGAGGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC201181 protein sequence
Red=Cloning site Green=Tags(s) MAKKVAVIGAGVSGLSSIKCCVDEDLEPTCFERSDDIGGLWKFTESSKDGMTRVYKSLVTNVCKEMSCYS DFPFHEDYPNFMNHEKFWDYLQEFAEHFDLLKYIQFKTTVCSITKRPDFSETGQWDVVTETEGKQNRAVF DAVMVCTGHFLNPHLPLEAFPGIHKFKGQILHSQEYKIPEGFQGKRVLVIGLGNTGGDIAVELSRTAAQV LLSTRTGTWVLGRSSDWGYPYNMMVTRRCCSFIAQVLPSRFLNWIQERKLNKRFNHEDYGLSITKGKKAK FIVNDELPNCILCGAITMKTSVIEFTETSAVFEDGTVEENIDVVIFTTGYTFSFPFFEEPLKSLCTKKIF LYKQVFPLNLERATLAIIGLIGLKGSILSGTELQARWVTRVFKGLCKIPPSQKLMMEATEKEQLIKRGVF KDTSKDKFDYIAYMDDIAACIGTKPSIPLLFLKDPRLAWEVFFGPCTPYQYRLMGPGKWDGARNAILTQW DRTLKPLKTRIVPDSSKPASMSHYLKAWGAPVLLASLLLICKSSLFLKLVRDKLQDRMSPYLVSLWRG SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002022 |
| ORF Size | 1674 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002022.3 |
| RefSeq Size | 2148 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 2329 |
| UniProt ID | P31512 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Drug metabolism - cytochrome P450 |
| MW | 63.3 kDa |
| Gene Summary | Metabolic N-oxidation of diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man. This results in a small subpopulation with reduced TMA N-oxidation capacity and causes fish odor syndrome (Trimethylaminuria). Three forms of the enzyme are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201181L3 | Lenti ORF clone of Human flavin containing monooxygenase 4 (FMO4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201181L4 | Lenti ORF clone of Human flavin containing monooxygenase 4 (FMO4), mGFP tagged |
CNY 5,890.00 |
|
| RG201181 | FMO4 (tGFP-tagged) - Human flavin containing monooxygenase 4 (FMO4) |
CNY 4,370.00 |
|
| SC122615 | FMO4 (untagged)-Human flavin containing monooxygenase 4 (FMO4) |
CNY 2,950.00 |
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