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EAP30 (SNF8) (NM_007241) Human Tagged ORF Clone
CAT＃: RC202137
SNF8 (Myc-DDK-tagged)-Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8)
ORF Plasmid: tGFP
"NM_007241" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dot3; EAP30; VPS22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202137 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCGCCGCGGGGTGGGAGCTGGCGCCATCGCCAAGAAGAAACTTGCAGAGGCCAAGTATAAGGAGC GAGGGACGGTCTTGGCTGAGGACCAGCTAGCCCAGATGTCAAAGCAGTTGGACATGTTCAAGACCAACCT GGAGGAATTTGCCAGCAAACACAAGCAGGAGATCCGGAAGAATCCTGAGTTCCGTGTGCAGTTCCAGGAC ATGTGTGCAACCATTGGCGTGGATCCGCTGGCCTCTGGAAAAGGATTTTGGTCTGAGATGCTGGGCGTGG GGGACTTCTATTACGAACTAGGTGTCCAAATTATCGAAGTGTGCCTGGCGCTGAAGCATCGGAATGGAGG TCTGATAACTTTGGAGGAACTACATCAACAGGTGTTGAAGGGAAGGGGCAAGTTCGCCCAGGATGTCAGT CAAGATGACCTGATCAGAGCCATCAAGAAACTAAAGGCACTTGGCACTGGCTTCGGCATCATCCCTGTGG GCGGCACTTACCTCATTCAGTCTGTTCCAGCTGAGCTCAATATGGATCACACCGTGGTGCTGCAGCTGGC AGAGAAGAATGGCTACGTGACTGTCAGTGAGATCAAAGCCAGTCTTAAATGGGAGACCGAGCGAGCGCGG CAAGTGCTGGAACACCTGCTGAAGGAAGGGTTGGCGTGGCTGGACTTACAGGCCCCAGGGGAGGCCCACT ACTGGCTGCCAGCTCTCTTCACTGACCTCTACTCCCAGGAGATTACAGCTGAGGAGGCCAGAGAAGCCCT CCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202137 protein sequence
Red=Cloning site Green=Tags(s) MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQD MCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVS QDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERAR QVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007241 |
| ORF Size | 774 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007241.4 |
| RefSeq Size | 1226 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 11267 |
| UniProt ID | Q96H20 |
| Domains | EAP30 |
| Protein Families | Transcription Factors |
| Protein Pathways | Endocytosis |
| MW | 28.9 kDa |
| Gene Summary | The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202137L1 | Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202137L2 | Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), mGFP tagged |
CNY 5,890.00 |
|
| RC202137L3 | Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202137L4 | Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), mGFP tagged |
CNY 5,890.00 |
|
| RG202137 | SNF8 (tGFP-tagged) - Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8) |
CNY 4,000.00 |
|
| SC115610 | SNF8 (untagged)-Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8) |
CNY 2,400.00 |
|
| SC322220 | SNF8 (untagged)-Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8) |
CNY 2,400.00 |
