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VPS25 (NM_032353) Human Tagged ORF Clone
CAT＃: RC202487
VPS25 (Myc-DDK-tagged)-Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25)
ORF Plasmid: tGFP
"NM_032353" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DERP9; EAP20; FAP20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202487 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTATCGCTTCCCACCCTTCTTTACGTTACAACCGAATGTGG ACACTCGGCAGAAGCAGCTGGCCGCCTGGTGCTCGCTGGTCCTGTCCTTCTGCCGCCTGCACAAACAGTC CAGCATGACGGTGATGGAAGCTCAGGAGAGCCCGCTCTTCAACAACGTCAAGCTACAGCGAAAGCTTCCT GTGGAGTCGATCCAGATTGTATTAGAGGAACTGAGGAAGAAAGGGAACCTCGAGTGGTTGGATAAGAGCA AGTCCAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATCTATCAGTGGGTTTCCAG GAGTGGCCAGAACAACTCCGTCTTTACCCTGTATGAACTGACTAATGGGGAAGACACAGAGGATGAGGAG TTCCACGGGCTGGATGAAGCCACTCTACTGCGGGCTCTGCAGGCCCTACAGCAGGAGCACAAGGCCGAGA TCATCACTGTCAGCGATGGCCGAGGCGTCAAGTTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202487 protein sequence
Red=Cloning site Green=Tags(s) MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP VESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEE FHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032353 |
| ORF Size | 528 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032353.4 |
| RefSeq Size | 1120 bp |
| RefSeq ORF | 531 bp |
| Locus ID | 84313 |
| UniProt ID | Q9BRG1 |
| Protein Families | Transcription Factors |
| Protein Pathways | Endocytosis |
| MW | 20.7 kDa |
| Gene Summary | This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202487L3 | Lenti ORF clone of Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202487L4 | Lenti ORF clone of Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25), mGFP tagged |
CNY 5,890.00 |
|
| RG202487 | VPS25 (tGFP-tagged) - Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25) |
CNY 4,000.00 |
|
| SC105402 | VPS25 (untagged)-Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25) |
CNY 2,400.00 |
