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CNOT10 (NM_015442) Human Tagged ORF Clone
CAT＃: RC202651
CNOT10 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 10 (CNOT10)
ORF Plasmid: tGFP
"NM_015442" in other vectors (4)
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CNY 8,176.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202651 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGACAAGCCTGCAGATCAGGGAGCAGAGAAACATGAAGGCACAGGTCAGTCCTCTGGGATCA CTGATCAAGAGAAGGAGTTATCCACCAATGCTTTCCAAGCTTTCACATCTGGAAATTATGATGCCTGTCT ACAACACCTTGCCTGTCTACAAGATATAAACAAAGATGATTATAAAATAATTTTGAATACAGCAGTAGCT GAGTTTTTTAAAAGTAACCAAACAACAACAGATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGG TCCACTCAGCTGTTGAAGAAATGGATGGATTAGATGATGTTGAAAACAGCATGTTGTACTATAATCAAGC AGTCATTCTTTATCATCTGCGGCAGTATACAGAAGCCATATCAGTTGGTGAAAAACTTTATCAGTTCATA GAGCCTTTTGAAGAAAAATTTGCCCAAGCAGTGTGTTTTTTGCTTGTAGACCTGTATATATTAACCTACC AAGCTGAGAAAGCTTTGCATCTTCTTGCTGTCCTAGAAAAAATGATTTCACAGGGTAACAATAACAAAAA TGGAAAGAATGAGACTGGTAATAACAACAACAAAGATGGATCTAATCATAAAGCTGAAAGTGGAGCTCTA ATAGAAGCTGCAAAATCAAAGATACATCAGTACAAAGTACGAGCTTATATCCAAATGAAGTCTCTGAAAG CATGTAAAAGGGAAATCAAGTCAGTCATGAATACAGCTGGAAATTCCGCACCCTCTCTCTTTCTTAAAAG CAATTTTGAGTACTTAAGAGGTAATTATCGAAAAGCCGTGAAGCTATTAAATAGTTCAAACATTGCTGAG CATCCAGGATTCATGAAAACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTTGGTTGCATCCATT TTGCCATGAGCAAGCACAATTTGGGAATATTCTACTTTAAAAAGGCTCTGCAAGAGAATGACAATGTCTG TGCACAGCTCAGTGCAGGTAGCACTGATCCAGGTAAAAAATTTTCAGGAAGACCCATGTGTACGTTACTA ACCAATAAGAGATATGAGTTGCTGTATAACTGTGGAATTCAGCTTCTTCACATTGGAAGGCCTCTTGCTG CCTTCGAATGTCTGATTGAAGCTGTTCAGGTTTATCATGCAAATCCTCGCCTCTGGCTACGGCTGGCTGA ATGCTGCATTGCTGCCAATAAGGGGACTTCTGAACAAGAAACTAAAGGCCTTCCCAGCAAAAAAGGAATT GTACAGTCTATTGTTGGTCAAGGCTATCATCGTAAAATAGTTTTGGCATCACAGTCTATACAGAATACTG TTTATAATGATGGTCAGTCTTCGGCCATTCCTGTAGCCAGTATGGAGTTTGCAGCCATATGTCTCAGAAA TGCCTTGTTGCTGCTACCTGAAGAACAGCAAGATCCAAAGCAGGAAAATGGGGCTAAAAATAGTAATCAA TTAGGTGGGAACACAGAGAGCAGCGAAAGCAGTGAAACTTGCAGCAGTAAAAGCCATGATGGAGATAAAT TCATTCCAGCTCCACCTTCTTCTCCATTGAGAAAACAGGAATTAGAAAACTTAAAGTGCTCCATACTTGC TTGCAGTGCCTACGTGGCTCTGGCTTTGGGTGATAACCTCATGGCTTTGAATCATGCAGATAAACTTCTT CAGCAGCCCAAGCTGTCAGGATCTCTTAAGTTTTTGGGACATTTATATGCTGCAGAAGCCCTCATCTCTC TCGACAGAATATCTGATGCCATTACTCACTTGAACCCGGAGAATGTCACTGATGTCTCCTTAGGGATCTC TTCAAATGAGCAGGACCAAGGATCAGACAAAGGTGAAAATGAAGCAATGGAATCCTCTGGTAAGCGGGCC CCTCAGTGCTACCCCAGTTCCGTCAACTCTGCCAGGACTGTGATGCTGTTCAACCTTGGCAGCGCTTACT GCCTGAGGAGCGAATATGACAAAGCCCGAAAGTGTCTCCACCAGGCGGCTTCAATGATCCATCCTAAAGA GGTGCCCCCTGAGGCCATCTTGCTGGCAGTCTACCTTGAACTGCAGAATGGTAATACTCAGCTGGCCTTA CAGATCATCAAAAGGAATCAGCTGCTCCCTGCAGTGAAAACACACTCTGAAGTGAGAAAGAAGCCAGTGT TTCAGCCTGTCCACCCGATCCAGCCCATCCAAATGCCGGCTTTCACCACTGTGCAGAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202651 protein sequence
Red=Cloning site Green=Tags(s) MAADKPADQGAEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVA EFFKSNQTTTDNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFI EPFEEKFAQAVCFLLVDLYILTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGAL IEAAKSKIHQYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAE HPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGSTDPGKKFSGRPMCTLL TNKRYELLYNCGIQLLHIGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPSKKGI VQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQ LGGNTESSESSETCSSKSHDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADKLL QQPKLSGSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRA PQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLAL QIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMPAFTTVQRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015442 |
| ORF Size | 2232 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015442.3 |
| RefSeq Size | 2829 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 25904 |
| UniProt ID | Q9H9A5 |
| Domains | TPR |
| Protein Pathways | RNA degradation |
| MW | 82.3 kDa |
| Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202651L3 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 10 (CNOT10), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202651L4 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 10 (CNOT10), mGFP tagged |
CNY 5,890.00 |
|
| RG202651 | CNOT10 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 10 (CNOT10) |
CNY 4,370.00 |
|
| SC112646 | CNOT10 (untagged)-Human CCR4-NOT transcription complex, subunit 10 (CNOT10) |
CNY 8,184.00 |
