UBE2Z (NM_023079) Human Tagged ORF Clone

CAT#: RC203689

UBE2Z (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2Z (UBE2Z)

ORF Plasmid: DDK tGFP



  "NM_023079" in other vectors (5)

Need custom modification / cloning service?
Get a free quote

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


UBE2Z rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HOYS7; USE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203689 representing NM_023079
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATTTATAAGGAGCCTCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTC
ATGCATTGATCACAGGCCCATTTGACACTCCTTATGAAGGGGGTTTCTTCCTGTTCGTGTTTCGGTGTCC
GCCCGACTATCCCATCCACCCACCTCGGGTCAAACTGATGACAACGGGCAATAACACAGTGAGGTTTAAC
CCCAACTTCTACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCTGCCTGGAGCC
CAGCCCAGAGCATCTCCTCAGTGCTCATCTCTATCCAGTCCCTGATGACTGAGAACCCCTATCACAATGA
GCCCGGCTTTGAACAGGAGAGACATCCAGGAGACAGCAAAAACTATAATGAATGTATCCGGCACGAGACC
ATCAGAGTTGCAGTCTGTGACATGATGGAAGGAAAGTGTCCCTGTCCTGAACCCCTACGAGGGGTGATGG
AGAAGTCCTTTCTGGAGTATTACGACTTCTATGAGGTGGCCTGCAAAGATCGCCTGCACCTTCAAGGCCA
AACTATGCAGGACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACCAGTCCCTCTTGATGCGCCTGGGA
CTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGAATGCAGAAATGGACTCTGATAGCAGTTCAT
CTGGGACAGAGACAGACCTTCATGGGAGCCTGAGGGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203689 representing NM_023079
Red=Cloning site Green=Tags(s)

MSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRFN
PNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHET
IRVAVCDMMEGKCPCPEPLRGVMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLG
LIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_023079
ORF Size 738 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_023079.2, NP_075567.1
RefSeq Size 3101 bp
RefSeq ORF 1065 bp
Locus ID 65264
UniProt ID Q9H832
Domains UBCc
Protein Pathways Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis
MW 38 kDa
Gene Summary This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis. [provided by RefSeq, Feb 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...