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ARFGAP1 (NM_175609) Human Tagged ORF Clone
CAT＃: RC206987
ARFGAP1 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2
ORF Plasmid: tGFP
"NM_175609" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARF1GAP; HRIHFB2281 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206987 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAACGTTTGTTTTG AGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGGATCTGCCTGGAGTGCTCGGG GAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGCTCTGTTACTATGGACAAGTGGAAGGACATT GAGCTTGAGAAGATGAAAGCTGGTGGGAATGCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGATTACG ATCCTTGCTGGTCCTTGCAGGAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGC TCTGGCCGAAGGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGG ACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCGGACAAGGCTT TTGAAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGGGGAATCGCTACGTGGGGTTTGG GAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTCCTCAACAACGCCATGTCCTCCCTGTACTCGGGC TGGAGCAGCTTCACCACTGGAGCCAGCCGGTTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTTTGGAT CCCAAGCGAGTCAGAAGTTTTGGGGTCACAAGCAGCAGCCGGAGCCGGCGTCCGAGCTGGGCCACAGCCT GAACGAGAACGTCCTCAAGCCTGCGCAGGAGAAGGTGAAGGAGGGAAAGATTTTTGATGATGTCTCCAGT GGGGTCTCTCAGTTGGCGTCCAAGGGAGTCGGTAGTAAGGGATGGCGGGACGTCACCACCTTTTTTTCGG GGAAAGCAGAGGGCCCCTTGGACAGCCCCTCGGAGGGCCACAGTTATCAGAACAGCGGTCTGGACCACTT CCAAAACAGCAACATAGACCAGAGCTTCTGGGAGACCTTTGGAAGTGCTGAGCCCACCAAGACCCGCAAG TCCCCGAGCAGCGACAGCTGGACGTGCGCGGACACCTCCACCGAGAGGAGGAGCTCGGACAGCTGGGAGG TGTGGGGCTCGGCCTCCACCAACAGGAACAGCAACAGCGACGGCGGGGAGGGCGGGGAGGGCACCAAGAA GGCAGTGCCGCCGGCCGTGCCCACTGATGATGGCTGGGACAACCAGAACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206987 protein sequence
Red=Cloning site Green=Tags(s) MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDI ELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPR TLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSG WSSFTTGASRFASAAKEGATKFGSQASQKFWGHKQQPEPASELGHSLNENVLKPAQEKVKEGKIFDDVSS GVSQLASKGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRK SPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175609 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175609.3 |
| RefSeq Size | 3309 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 55738 |
| UniProt ID | Q8N6T3 |
| Protein Pathways | Endocytosis |
| MW | 45.7 kDa |
| Gene Summary | The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206987L3 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206987L4 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206987 | ARFGAP1 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2 |
CNY 4,370.00 |
|
| SC126067 | ARFGAP1 (untagged)-Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2 |
CNY 2,950.00 |
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