Pepsinogen II (PGC) (NM_002630) Human Tagged ORF Clone
CAT#: RC210051
PGC (Myc-DDK-tagged)-Human progastricsin (pepsinogen C) (PGC), transcript variant 1
ORF Plasmid: tGFP
"NM_002630" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PEPC; PGII |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210051 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTGGATGGTGGTGGTCTTGGTCTGCCTCCAGCTCTTGGAGGCAGCAGTGGTCAAAGTGCCCCTGA AGAAATTTAAGTCTATCCGTGAGACCATGAAGGAGAAGGGCTTGCTGGGGGAGTTCCTGAGGACCCACAA GTATGATCCTGCTTGGAAGTACCGCTTTGGTGACCTCAGCGTGACCTACGAGCCCATGGCCTACATGGAT GCTGCCTACTTTGGTGAGATCAGCATCGGGACTCCACCCCAGAACTTCCTGGTCCTTTTTGACACCGGCT CCTCCAACTTGTGGGTGCCCTCTGTCTACTGCCAGAGCCAGGCCTGCACCAGTCACTCCCGCTTCAACCC CAGCGAGTCGTCCACCTACTCCACCAATGGGCAGACCTTCTCCCTGCAGTATGGCAGTGGCAGCCTCACC GGCTTCTTTGGCTATGACACCCTGACTGTCCAGAGCATCCAGGTCCCCAACCAGGAGTTCGGCTTGAGTG AGAATGAGCCTGGTACCAACTTCGTCTATGCGCAGTTTGATGGCATCATGGGCCTGGCCTACCCTGCTCT GTCCGTGGATGAGGCCACCACAGCTATGCAGGGCATGGTGCAGGAGGGCGCCCTCACCAGCCCCGTCTTC AGCGTCTACCTCAGCAACCAGCAGGGCTCCAGCGGGGGAGCGGTTGTCTTTGGGGGTGTGGATAGCAGCC TGTACACGGGGCAGATCTACTGGGCGCCTGTCACCCAGGAACTCTACTGGCAGATTGGCATTGAAGAGTT CCTCATCGGCGGCCAGGCCTCCGGCTGGTGTTCTGAGGGTTGCCAGGCCATCGTGGACACAGGCACCTCT CTGCTCACTGTGCCCCAGCAGTACATGAGTGCTCTTCTGCAGGCCACAGGGGCCCAGGAGGATGAGTATG GACAGTTTCTCGTGAACTGTAACAGCATTCAGAATCTGCCCAGCTTGACCTTCATCATCAATGGTGTGGA GTTCCCTCTGCCACCTTCCTCCTATATCCTCAGTAACAACGGCTACTGCACCGTGGGAGTCGAGCCCACC TACCTGTCCTCCCAGAACGGCCAGCCCCTGTGGATCCTCGGGGATGTCTTCCTCAGGTCCTACTATTCCG TCTACGACTTGGGCAACAACAGAGTAGGCTTTGCCACTGCCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210051 protein sequence
Red=Cloning site Green=Tags(s) MKWMVVVLVCLQLLEAAVVKVPLKKFKSIRETMKEKGLLGEFLRTHKYDPAWKYRFGDLSVTYEPMAYMD AAYFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQSQACTSHSRFNPSESSTYSTNGQTFSLQYGSGSLT GFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPALSVDEATTAMQGMVQEGALTSPVF SVYLSNQQGSSGGAVVFGGVDSSLYTGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSEGCQAIVDTGTS LLTVPQQYMSALLQATGAQEDEYGQFLVNCNSIQNLPSLTFIINGVEFPLPPSSYILSNNGYCTVGVEPT YLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGFATAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002630 |
ORF Size | 1164 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002630.4 |
RefSeq Size | 1392 bp |
RefSeq ORF | 1167 bp |
Locus ID | 5225 |
UniProt ID | P20142 |
Protein Families | Protease, Secreted Protein |
MW | 42.4 kDa |
Gene Summary | This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210051L3 | Lenti ORF clone of Human progastricsin (pepsinogen C) (PGC), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210051L4 | Lenti ORF clone of Human progastricsin (pepsinogen C) (PGC), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG210051 | PGC (tGFP-tagged) - Human progastricsin (pepsinogen C) (PGC), transcript variant 1 |
CNY 4,370.00 |
|
SC123989 | PGC (untagged)-Human progastricsin (pepsinogen C) (PGC), transcript variant 1 |
CNY 2,950.00 |
其它Pepsinogen II产品