Azurocidin (AZU1) (NM_001700) Human Tagged ORF Clone

CAT#: RC210151

AZU1 (Myc-DDK-tagged)-Human azurocidin 1 (AZU1)

ORF Plasmid: DDK tGFP



  "NM_001700" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


AZU1 Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR; NAZC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210151 representing NM_001700
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCGGCTGACAGTCCTGGCCCTGCTGGCTGGTCTGCTGGCGTCCTCGAGGGCCGGCTCCAGCCCCC
TTTTGGACATCGTTGGCGGCCGGAAGGCGAGGCCCCGCCAGTTCCCGTTCCTGGCCTCCATTCAGAATCA
AGGCAGGCACTTCTGCGGGGGTGCCCTGATCCATGCCCGCTTCGTGATGACCGCGGCCAGCTGCTTCCAA
AGCCAGAACCCCGGGGTTAGCACCGTGGTGCTGGGTGCCTATGACCTGAGGCGGCGGGAGAGGCAGTCCC
GCCAGACGTTTTCCATCAGCAGCATGAGCGAGAATGGCTACGACCCCCAGCAGAACCTGAACGACCTGAT
GCTGCTTCAGCTGGACCGTGAGGCCAACCTCACCAGCAGCGTGACGATACTGCCACTGCCTCTGCAGAAC
GCCACGGTGGAAGCCGGCACCAGATGCCAGGTGGCCGGCTGGGGGAGCCAGCGCAGTGGGGGGCGTCTCT
CCCGTTTTCCCAGGTTTGTCAACGTGACTGTGACCCCCGAGGACCAGTGTCGCCCCAACAACGTGTGCAC
CGGTGTGCTCACCCGCCGCGGTGGCATCTGCAATGGGGACGGGGGCACCCCCCTCGTCTGCGAGGGCCTG
GCCCACGGCGTGGCCTCCTTTTCCCTGGGGCCCTGTGGCCGAGGCCCTGACTTCTTCACCCGAGTGGCGC
TCTTCCGAGACTGGATCGATGGTGTTCTCAACAACCCGGGACCGGGGCCAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210151 representing NM_001700
Red=Cloning site Green=Tags(s)

MTRLTVLALLAGLLASSRAGSSPLLDIVGGRKARPRQFPFLASIQNQGRHFCGGALIHARFVMTAASCFQ
SQNPGVSTVVLGAYDLRRRERQSRQTFSISSMSENGYDPQQNLNDLMLLQLDREANLTSSVTILPLPLQN
ATVEAGTRCQVAGWGSQRSGGRLSRFPRFVNVTVTPEDQCRPNNVCTGVLTRRGGICNGDGGTPLVCEGL
AHGVASFSLGPCGRGPDFFTRVALFRDWIDGVLNNPGPGPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001700
ORF Size 753 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001700.5
RefSeq Size 912 bp
RefSeq ORF 756 bp
Locus ID 566
UniProt ID P20160
Domains Tryp_SPc
Protein Families Druggable Genome, Protease
MW 26.89 kDa
Gene Summary Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. This gene encodes a preproprotein that is proteolytically processed to generate a mature azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Nov 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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