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PIK3CB (NM_006219) Human Tagged ORF Clone
CAT＃: RC215433
- TrueORF®
PIK3CB (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB)
ORF Plasmid: tGFP
"NM_006219" in other vectors (6)
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CNY 7,448.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P110BETA; PI3K; PI3KBETA; PIK3C1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215433 representing NM_006219
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTTCAGTTTCATAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGA TAGCATCTGATGGCTCCATACCTGTGGATTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACC TCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCCAATGTTCAAC CTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAG ATGAAACACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGA CCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTG AAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCAGCGAGGAAAAAATCCTGTCACTTG TGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTGAAAA CTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTT AGCTTTCAAGTGTCTCCTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTA TTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATTATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGT TTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACAGAGCCCTGCCCCAT TTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCA TAAATCGAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTG GGAAAATAACAACCCTTTCCAAATTGTCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAA GTTCATGTCAGGGCTGGTCTTTTTCATGGTACTGAGCTCCTGTGTAAAACCATCGTAAGCTCAGAGGTAT CAGGGAAAAATGATCATATTTGGAATGAACCACTGGAATTTGATATTAATATTTGTGACTTACCAAGAAT GGCTCGATTATGTTTTGCTGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAACGAAAACTATT AATCCCTCTAAATATCAGACCATCAGGAAAGCTGGAAAAGTGCATTATCCTGTAGCGTGGGTAAATACGA TGGTTTTTGACTTTAAAGGACAATTGAGAACTGGAGACATAATATTACACAGCTGGTCTTCATTTCCTGA TGAACTCGAAGAAATGTTGAATCCAATGGGAACTGTTCAAACAAATCCATATACTGAAAATGCAACAGCT TTGCATGTTAAATTTCCAGAGAATAAAAAACAACCTTATTATTACCCTCCCTTCGATAAGATTATTGAAA AGGCAGCTGAGATTGCAAGCAGTGATAGTGCTAATGTGTCAAGTCGAGGTGGAAAAAAGTTTCTTCCTGT ATTGAAAGAAATCTTGGACAGGGATCCCTTGTCTCAACTGTGTGAAAATGAAATGGATCTTATTTGGACT TTGCGACAAGACTGCCGAGAGATTTTCCCACAATCACTGCCAAAATTACTGCTGTCAATCAAGTGGAATA AACTTGAGGATGTTGCTCAGCTTCAGGCGCTGCTTCAGATTTGGCCTAAACTGCCCCCCCGGGAGGCCCT AGAGCTTCTGGATTTCAACTATCCAGACCAGTACGTTCGAGAATATGCTGTAGGCTGCCTGCGACAGATG AGTGATGAAGAACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAGCCTTTTCTTGATT GTGCCCTCTCTAGATTCCTATTAGAAAGAGCACTTGGTAATCGGAGGATAGGGCAGTTTCTATTTTGGCA TCTTAGGTCAGAAGTGCACATTCCTGCTGTCTCAGTACAATTTGGTGTCATCCTTGAAGCATACTGCCGG GGAAGTGTGGGGCACATGAAAGTGCTTTCTAAGCAGGTTGAAGCACTCAATAAGTTAAAAACTTTAAATA GTTTAATCAAACTGAATGCCGTGAAGTTAAACAGAGCCAAAGGGAAGGAGGCCATGCATACCTGTTTAAA ACAGAGTGCTTACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCATGTGTTATCCTCTCAGAA CTCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGAAGCCTTTGTGGCTGGTATACAATAACAAGG TATTTGGTGAGGATTCAGTTGGAGTGATTTTTAAAAATGGTGATGATTTACGACAGGATATGTTGACACT CCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTGGTTTGGATCTTCGGATGTTGCCTTATGGC TGTTTAGCAACAGGAGATCGCTCTGGCCTCATTGAAGTTGTGAGCACCTCTGAAACAATTGCTGACATTC AGCTGAACAGTAGCAATGTGGCTGCTGCAGCAGCCTTCAACAAAGATGCCCTTCTGAACTGGCTTAAAGA ATACAACTCTGGGGATGACCTGGACCGAGCCATTGAGGAATTTACACTGTCCTGTGCTGGCTACTGTGTA GCTTCTTATGTCCTTGGGATTGGTGACAGACATAGTGACAACATCATGGTCAAAAAAACTGGCCAGCTCT TCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAGTTTGGCATTAAAAGGGAGCGAGTGCC TTTTATTCTTACCTATGATTTCATCCATGTCATTCAACAAGGAAAAACAGGAAATACAGAAAAGTTTGGC CGGTTCCGCCAGTGTTGTGAGGATGCATATCTGATTTTACGACGGCATGGGAATCTCTTCATCACTCTCT TTGCGCTGATGTTGACTGCAGGGCTTCCTGAACTCACATCAGTCAAAGATATACAGTATCTTAAGGACTC TCTTGCATTAGGGAAGAGTGAAGAAGAAGCACTCAAACAGTTTAAGCAAAAATTTGATGAGGCGCTCAGG GAAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACAGTTCGGAAAGACTACAGATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215433 representing NM_006219
Red=Cloning site Green=Tags(s) MCFSFIMPPAMADILDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFN LLMDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGKGLHEFDSL KDPEVNEFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVF SFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRALPH FILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVK VHVRAGLFHGTELLCKTIVSSEVSGKNDHIWNEPLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTI NPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATA LHVKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILDRDPLSQLCENEMDLIWT LRQDCREIFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQM SDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCR GSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSE LYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYG CLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCV ASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFG RFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALR ESWTTKVNWMAHTVRKDYRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006219 |
| ORF Size | 3210 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006219.3 |
| RefSeq Size | 3213 bp |
| RefSeq ORF | 3213 bp |
| Locus ID | 5291 |
| UniProt ID | P42338 |
| Domains | PI3K_rbd, PI3_PI4_kinase, PI3Ka, PI3K_C2, PI3K_p85B |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway |
| MW | 122.6 kDa |
| Gene Summary | This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-altered prostate cancers
,null,
Nature Communications
,PubMed ID 34417459
[PIK3CB]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215433L1 | Lenti ORF clone of Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB), Myc-DDK-tagged |
CNY 9,848.00 |
|
| RC215433L2 | Lenti ORF clone of Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB), mGFP tagged |
CNY 10,260.00 |
|
| RC215433L3 | Lenti ORF clone of Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC215433L4 | Lenti ORF clone of Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB), mGFP tagged |
CNY 9,848.00 |
|
| RG215433 | PIK3CB (tGFP-tagged) - Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB) |
CNY 9,220.00 |
|
| SC116228 | PIK3CB (untagged)-Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB) |
CNY 7,456.00 |
