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Sphingosine 1 phosphate phosphatase 2 (SGPP2) (NM_152386) Human Tagged ORF Clone
CAT＃: RC217993
SGPP2 (Myc-DDK-tagged)-Human sphingosine-1-phosphate phosphatase 2 (SGPP2)
ORF Plasmid: tGFP
"NM_152386" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SPP2; SPPase2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217993 representing NM_152386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGCTGCTGCGGAGCCTGCAGGATTCCCAGCTCGTCGCCCGCTTCCAGCGCCGCTGCGGGCTCT TCCCCGCTCCGGATGAAGGCCCCCGGGAGAACGGCGCGGACCCCACGGAGCGCGCGGCGCGGGTCCCCGG GGTCGAGCATCTCCCCGCAGCCAACGGCAAGGGCGGCGAGGCTCCGGCCAACGGGCTGCGCAGAGCCGCG GCGCCGGAGGCTTATGTACAGAAGTACGTCGTGAAGAATTATTTCTACTATTACCTATTCCAATTTTCAG CTGCTTTGGGCCAAGAAGTGTTCTACATCACGTTTCTTCCATTCACTCACTGGAATATTGACCCTTATTT ATCCAGAAGATTGATCATCATATGGGTTTTGGTGATGTATATTGGCCAAGTGGCCAAGGATGTCTTGAAG TGGCCCCGTCCCTCCTCCCCTCCAGTTGTAAAACTGGAAAAGAGACTGATCGCTGAATATGGAATGCCAT CCACCCACGCCATGGCGGCCACTGCCATTGCCTTCACCCTCCTTATCTCTACTATGGACAGATACCAGTA TCCATTTGTGTTGGGACTGGTGATGGCCGTGGTGTTTTCCACCTTGGTGTGTCTCAGCAGGCTCTACACT GGGATGCATACGGTCCTGGATGTGCTGGGTGGCGTCCTGATCACCGCACTCCTCATCGTCCTCACCTACC CTGCCTGGACCTTCATCGACTGCCTGGACTCGGCCAGCCCCCTCTTCCCCGTGTGTGTCATAGTTGTGCC ATTCTTCCTGTGTTACAATTACCCTGTTTCTGATTACTACAGCCCAACCCGGGCGGACACCACCACCATT CTGGCTGCCGGGGCTGGAGTGACCATAGGATTCTGGATCAACCATTTCTTCCAGCTTGTATCCAAGCCCG CTGAATCTCTCCCTGTTATTCAGAACATCCCACCACTCACCACCTACATGTTAGTTTTGGGTCTGACCAA ATTTGCAGTGGGAATTGTGTTGATCCTCTTGGTTCGTCAGCTTGTACAAAATCTCTCACTGCAAGTATTA TACTCATGGTTCAAGGTGGTCACCAGGAACAAGGAGGCCAGGCGGAGACTGGAGATTGAAGTGCCTTACA AGTTTGTTACCTACACATCTGTTGGCATCTGCGCTACAACCTTTGTGCCGATGCTTCACAGGTTTCTGGG ATTACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217993 representing NM_152386
Red=Cloning site Green=Tags(s) MAELLRSLQDSQLVARFQRRCGLFPAPDEGPRENGADPTERAARVPGVEHLPAANGKGGEAPANGLRRAA APEAYVQKYVVKNYFYYYLFQFSAALGQEVFYITFLPFTHWNIDPYLSRRLIIIWVLVMYIGQVAKDVLK WPRPSSPPVVKLEKRLIAEYGMPSTHAMAATAIAFTLLISTMDRYQYPFVLGLVMAVVFSTLVCLSRLYT GMHTVLDVLGGVLITALLIVLTYPAWTFIDCLDSASPLFPVCVIVVPFFLCYNYPVSDYYSPTRADTTTI LAAGAGVTIGFWINHFFQLVSKPAESLPVIQNIPPLTTYMLVLGLTKFAVGIVLILLVRQLVQNLSLQVL YSWFKVVTRNKEARRRLEIEVPYKFVTYTSVGICATTFVPMLHRFLGLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152386 |
| ORF Size | 1197 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152386.4 |
| RefSeq Size | 1200 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 130367 |
| UniProt ID | Q8IWX5 |
| Protein Families | Transmembrane |
| Protein Pathways | Sphingolipid metabolism |
| MW | 44.6 kDa |
| Gene Summary | The protein encoded by this gene is a transmembrane protein that degrades the bioactive signaling molecule sphingosine 1-phosphate. The encoded protein is induced during inflammatory responses and has been shown to be downregulated by the microRNA-31 tumor suppressor. Alternative splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217993L3 | Lenti ORF clone of Human sphingosine-1-phosphate phosphatase 2 (SGPP2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217993L4 | Lenti ORF clone of Human sphingosine-1-phosphate phosphatase 2 (SGPP2), mGFP tagged |
CNY 5,890.00 |
|
| RG217993 | SGPP2 (tGFP-tagged) - Human sphingosine-1-phosphate phosphatase 2 (SGPP2) |
CNY 4,370.00 |
|
| SC306384 | SGPP2 (untagged)-Human sphingosine-1-phosphate phosphatase 2 (SGPP2) |
CNY 3,656.00 |
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