RP1 (NM_006269) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DCDC4A; ORP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC219283 representing NM_006269 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGATACCCCTTCTACTGGTTTTTCCATCATTCATCCTACGTCTTCTGAAGGTCAAGTTCCACCCC CTCGCCATTTGAGCCTCACTCATCCTGTTGTGGCCAAGCGAATCAGTTTCTACAAGAGCGGAGACCCCCA ATTCGGCGGGGTCAGGGTGGTGGTCAACCCTCGCTCCTTTAAGTCCTTTGATGCTCTGCTGGATAACTTG TCCAGGAAGGTGCCCCTCCCTTTTGGAGTGAGGAACATCAGCACCCCTCGGGGCAGGCACAGCATCACGC GCCTGGAGGAGCTGGAGGACGGCGAGTCCTACCTATGTTCCCACGGCAGGAAGGTGCAGCCTGTAGACCT GGACAAAGCCCGTCGGCGCCCGCGGCCCTGGCTCAGCAGCCGGGCCATTAGCGCGCACTCACCGCCCCAC CCCGTAGCCGTCGCTGCTCCCGGCATGCCCCGCCCCCCACGGAGCCTAGTGGTCTTCAGGAATGGCGACC CGAAGACGAGGCGTGCGGTTCTTCTGAGCAGGAGGGTCACCCAGAGCTTCGAGGCATTTCTACAGCACCT GACAGAGGTCATGCAGCGCCCTGTGGTCAAGCTGTACGCTACGGACGGAAGGAGGGTTCCCAGCCTCCAG GCAGTGATCCTGAGCTCTGGAGCTGTGGTGGCGGCAGGAAGGGAGCCATTTAAACCAGGAAATTATGACA TCCAAAAATACTTGCTTCCTGCTAGATTACCAGGGATCTCTCAGCGTGTGTACCCCAAGGGAAATGCAAA GTCAGAAAGCAGAAAGATAAGCACACATATGTCTTCAAGCTCAAGGTCCCAGATTTATTCTGTTTCTTCT GAGAAAACACATAATAATGATTGCTACTTAGACTATTCTTTTGTTCCTGAAAAGTACTTGGCCTTAGAAA AGAATGATTCTCAGAATTTACCAATATATCCTTCTGAAGATGATATTGAGAAATCAATTATTTTTAATCA AGACGGCACTATGACAGTTGAGATGAAAGTTCGATTCAGAATAAAAGAGGAAGAAACCATAAAATGGACA ACTACTGTCAGTAAAACTGGTCCTTCTAATAATGATGAAAAGAGTGAGATGAGTTTTCCAGGAAGAACAG AAAGTCGATCATCTGGTTTAAAGCTTGCAGCATGTTCATTCTCTGCAGATGTGTCACCTATGGAGCGAAG CAGTAATCAAGAGGGCAGTTTGGCAGAGGAGATAAACATTCAAATGACAGATCAAGTGGCTGAAACTTGC AGTTCTGCTAGTTGGGAGAATGCTACTGTGGACACAGATATCATCCAGGGAACTCAAGACCAAGCAAAGC ATCGTTTTTATAGGCCCCCTACACCTGGACTAAGAAGAGTGAGACAAAAGAAATCTGTGATTGGCAGTGT GACCTTAGTATCTGAAACTGAGGTTCAAGAGAAAATGATTGGACAGTTTTCATATAGTGAAGAAAGGGAA AGTGGGGAAAACAAGTCTGAGTATCACATGTTTACACATTCTTGCAGTAAAATGTCATCAGTATCTAACA AACCAGTACTTGTTCAGATCAATAACAATGATCAAATGGAGGAGTCATCATTAGAAAGAAAAAAGGAAAA CAGTCTGCTTAAGTCAAGTGCAATAAGTGCTGGTGTTATAGAAATTACAAGTCAGAAGATGTTAGAGATG TCACATAATAATGGTTTGCCATCAACTATATCAAATAACTCAATTGTGGAGGAAGATGTAGTTGATTGTG TGGTATTGGACAACAAAACTGGTATCAAGAACTTCAAAACTTATGGTAACACCAATGATAGGTTCAGTCC TATTTCAGCAGATGCAACCCATTTTTCAAGTAATAACTCTGGAACTGACAAAAATATTTCTGAGGCTCCA GCTTCAGAAGCATCCTCTACTGTCACTGCAAGAATTGACAGACTAATTAATGAATTTGCTCAGTGTGGTT TAACAAAACTTCCAAAAAATGAAAAGAAGATTTTGTCATCTGTTGCCAGCAAAAAGAAGAAAAAATCTCG ACAGCAAGCAATAAATTCCAGGTATCAAGATGGACAGCTTGCAACCAAAGGAATTCTTAATAAGAATGAG AGAATAAACACAAAAGGTAGAATTACAAAGGAAATGATAGTGCAAGATTCAGATAGTCCCCTTAAAGGAG GGATACTTTGTGAGGAAGACCTCCAGAAAAGTGATACTGTAATTGAATCAAATACTTTTTGTTCCAAAAG TAATCTCAATTCCACGATTTCCAAGAATTTCCATAGAAATAAATTAAATACTACTCAAAATTCCAAGGTT CAAGGACTTTTAACCAAAAGAAAATCTAGATCACTAAATAAAATAAGCTTAGGAGCACCTAAAAAAAGAG AAATCGGTCAAAGAGATAAAGTGTTTCCTCACAATGAATCTAAATATTGCAAAAGTACTTTTGAAAACAA AAGTTTATTTCATGTATTTAACATCCTTGAGCAAAAACCCAAAGATTTTTATGCACCGCAATCTCAAGCA GAAGTGGCATCTGGGTATTTGAGAGGAATGGCAAAGAAGAGTTTAGTTTCAAAAGTTACTGATTCACACA TAACTTTAAAAAGCCAGAAAAAACGTAAAGGGGATAAAGTGAAAGCAAGTGCTATTTTAAGTAAACAACA TGCTACAACCAGGGCAAATTCTTTAGCTTCTTTGAAAAAACCTGATTTTCCTGAGGCTATTGCTCATCAT TCAATTCAAAATTATATACAGAGTTGGTTGCAGAACATAAATCCATATCCAACTTTAAAGCCTATAAAAT CAGCTCCAGTATGTAGAAATGAAACGAGTGTGGTAAATTGTAGCAATAATAGTTTTTCAGGGAATGATCC CCATACAAATTCTGGAAAAATAAGTAATTTTGTTATGGAAAGTAATAAGCACATAACTAAAATTGCCGGT TTGACAGGAGATAATCTATGTAAAGAGGGAGATAAGTCTTTTATTGCCAATGACACTGGTGAAGAAGATC TCCATGAGACACAGGTTGGATCTCTGAATGATGCTTATTTGGTTCCCCTGCATGAACACTGTACTTTGTC ACAGTCAGCTATTAATGATCATAATACTAAAAGTCATATAGCTGCTGAAAAATCAGGACCAGAGAAAAAA CTTGTTTACCAGGAAATAAACCTAGCTAGAAAAAGGCAAAGTGTAGAGGCTGCCATTCAAGTAGATCCTA TAGAAGAGGAAACTCCAAAAGACCTCTTACCAGTCCTGATGCTTCACCAATTGCAAGCTTCAGTTCCTGG TATTCACAAGACTCAGAATGGAGTTGTTCAAATGCCAGGTTCACTTGCAGGTGTTCCCTTTCATTCTGCA ATATGTAATTCATCCACTAATCTCCTTCTAGCTTGGCTCTTGGTGCTAAACCTAAAGGGAAGTATGAATA GCTTCTGTCAAGTTGATGCTCACAAGGCTACCAACAAATCTTCAGAAACACTTGCATTGTTGGAGATTCT AAAGCACATAGCTATCACAGAGGAAGCTGATGACTTGAAAGCTGCTGTTGCCAATTTAGTGGAGTCAACT ACAAGCCACTTTGGACTCAGTGAGAAAGAACAAGACATGGTTCCAATAGATCTTTCTGCAAATTGTTCCA CGGTCAACATTCAGAGTGTTCCTAAGTGCAGTGAAAATGAAAGAACACAAGGAATCTCCTCTTTGGATGG AGGTTGCTCTGCCAGTGAGGCATGTGCCCCTGAAGTCTGTGTTTTGGAAGTGACTTGCTCTCCATGTGAG ATGTGCACTGTAAATAAGGCTTATTCTCCAAAAGAGACATGTAACCCCAGTGACACTTTTTTTCCTAGTG ATGGTTATGGTGTGGATCAGACTTCTATGAATAAGGCTTGTTTCCTAGGAGAGGTCTGTTCACTTACTGA TACTGTGTTTTCTGATAAGGCTTGTGCTCAAAAGGAGAACCATACCTATGAGGGAGCTTGCCCAATTGAT GAGACCTACGTTCCTGTCAATGTCTGCAATACCATTGACTTTTTAAACTCCAAAGAAAACACATATACTG ATAACTTGGATTCAACTGAAGAGTTAGAAAGAGGTGATGACATTCAGAAAGATCTAAATATTTTGACAGA CCCTGAATATAAAAATGGATTTAATACATTGGTGTCACATCAAAATGTCAGTAATTTAAGCTCCTGTGGC CTTTGCCTAAGTGAAAAAGAAGCAGAACTTGATAAGAAACATAGTTCTCTAGATGATTTTGAAAATTGTT CACTAAGGAAGTTTCAGGATGAAAATGCATATACTTCCTTTGATATGGAAGAACCACGGACTTCTGAAGA ACCAGGCTCAATAACCAACAGCATGACATCAAGTGAAAGAAACATTTCAGAATTGGAATCTTTTGAAGAA TTAGAAAACCATGACACTGATATCTTTAATACAGTGGTAAATGGAGGAGAGCAAGCCACTGAAGAATTAA TCCAAGAAGAGGTAGAGGCTAGTAAAACTTTAGAATTGATAGACATCTCTAGTAAGAATATTATGGAAGA AAAAAGAATGAACGGTATAATTTATGAAATAATCAGTAAGAGGCTGGCAACACCACCATCTTTAGATTTT TGCTATGATTCTAAGCAAAATAGTGAAAAGGAGACCAATGAAGGAGAAACTAAGATGGTAAAAATGATGG TGAAAACTATGGAAACTGGAAGTTATTCAGAGTCCTCTCCTGATTTAAAAAAATGCATCAAAAGTCCAGT GACTTCTGATTGGTCAGACTATCGGCCTGACAGTGACAGTGAGCAGCCATATAAAACATCCAGTGATGAT CCCAATGACAGTGGCGAACTTACCCAAGAGAAAGAATATAACATAGGATTTGTTAAAAGGGCAATAGAAA AACTGTACGGTAAAGCAGATATTATCAAACCATCTTTTTTTCCTGGGTCTACCCGCAAATCTCAGGTTTG TCCTTATAATTCTGTGGAATTTCAGTGTTCCAGGAAAGCAAGTCTTTATGATTCTGAAGGGCAGTCATTT GGCTCTTCTGAACAGGTATCTAGTAGTTCATCTATGTTGCAGGAATTCCAGGAGGAAAGACAAGATAAGT GTGATGTTAGTGCTGTGAGGGACAATTATTGTAGGGGTGACATTGTAGAACCTGGTACAAAACAAAATGA TGATAGCAGAATCCTCACAGACATAGAGGAAGGAGTACTGATTGACAAAGGCAAATGGCTTCTGAAAGAA AATCATTTGCTAAGGATGTCATCTGAAAATCCTGGCATGTGTGGCAATGCAGACACCACATCAGTGGACA CCCTACTTGATAATAACAGCAGTGAGGTACCATATTCACATTTTGGTAATTTGGCCCCAGGCCCAACGAT GGATGAACTCTCCTCTTCAGAACTCGAGGAACTGACTCAACCCCTTGAACTAAAATGCAATTACTTTAAC ATGCCTCATGGTAGTGACTCAGAACCTTTTCATGAGGACTTGCTGGATGTTCGCAATGAAACCTGTGCCA AGGAAAGAATAGCAAATCATCATACAGAGGAGAAGGGTAGTCATCAGTCAGAAAGAGTATGCACATCTGT CACTCATTCCTTTATTTCTGCTGGTAACAAAGTCTACCCTGTCTCTGATGATGCTATTAAAAACCAACCA TTGCCTGGCAGTAATATGATTCATGGTACACTTCAGGAAGCTGACTCTTTGGATAAACTGTATGCTCTTT GTGGTCAACATTGCCCAATACTAACTGTTATTATCCAACCCATGAATGAGGAAGACCGAGGATTTGCATA TCGCAAAGAATCTGATATTGAAAATTTCTTGGGTTTTTATTTATGGATGAAAATACACCCATATTTACTT CAGACAGACAAAAATGTGTTCAGGGAAGAGAACAATAAAGCAAGTATGAGACAAAATCTTATTGATAATG CCATTGGTGATATATTTGATCAGTTTTATTTCAGTAACACATTTGACTTGATGGGTAAAAGAAGAAAACA AAAAAGAATTAACTTCTTGGGGTTAGAGGAAGAAGGTAATTTAAAGAAATTTCAACCAGATTTGAAGGAA AGGTTTTGTATGAATTTCTTGCACACATCATTGTTAGTTGTGGGTAATGTGGATTCAAATACACAAGACC TCAGCGGTCAGACAAATGAAATCTTTAAAGCAGTCGATGAGAATAACAACTTATTAAATAACAGATTCCA GGGCTCAAGAACAAATCTCAACCAAGTAGTAAGAGAAAATATCAACTGTCATTACTTCTTTGAAATGCTT GGTCAAGCTTGCCTCTTAGATATTTGCCAAGTTGAGACCTCCTTAAATATTAGCAACAGAAATATTTTAG AACTTTGTATGTTTGAGGGTGAAAATCTTTTCATTTGGGAAGAGGAAGACATATTAAATTTAACTGATCT TGAAAGCAGTAGAGAACAAGAAGATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219283 representing NM_006269 Red=Cloning site Green=Tags(s) MSDTPSTGFSIIHPTSSEGQVPPPRHLSLTHPVVAKRISFYKSGDPQFGGVRVVVNPRSFKSFDALLDNL SRKVPLPFGVRNISTPRGRHSITRLEELEDGESYLCSHGRKVQPVDLDKARRRPRPWLSSRAISAHSPPH PVAVAAPGMPRPPRSLVVFRNGDPKTRRAVLLSRRVTQSFEAFLQHLTEVMQRPVVKLYATDGRRVPSLQ AVILSSGAVVAAGREPFKPGNYDIQKYLLPARLPGISQRVYPKGNAKSESRKISTHMSSSSRSQIYSVSS EKTHNNDCYLDYSFVPEKYLALEKNDSQNLPIYPSEDDIEKSIIFNQDGTMTVEMKVRFRIKEEETIKWT TTVSKTGPSNNDEKSEMSFPGRTESRSSGLKLAACSFSADVSPMERSSNQEGSLAEEINIQMTDQVAETC SSASWENATVDTDIIQGTQDQAKHRFYRPPTPGLRRVRQKKSVIGSVTLVSETEVQEKMIGQFSYSEERE SGENKSEYHMFTHSCSKMSSVSNKPVLVQINNNDQMEESSLERKKENSLLKSSAISAGVIEITSQKMLEM SHNNGLPSTISNNSIVEEDVVDCVVLDNKTGIKNFKTYGNTNDRFSPISADATHFSSNNSGTDKNISEAP ASEASSTVTARIDRLINEFAQCGLTKLPKNEKKILSSVASKKKKKSRQQAINSRYQDGQLATKGILNKNE RINTKGRITKEMIVQDSDSPLKGGILCEEDLQKSDTVIESNTFCSKSNLNSTISKNFHRNKLNTTQNSKV QGLLTKRKSRSLNKISLGAPKKREIGQRDKVFPHNESKYCKSTFENKSLFHVFNILEQKPKDFYAPQSQA EVASGYLRGMAKKSLVSKVTDSHITLKSQKKRKGDKVKASAILSKQHATTRANSLASLKKPDFPEAIAHH SIQNYIQSWLQNINPYPTLKPIKSAPVCRNETSVVNCSNNSFSGNDPHTNSGKISNFVMESNKHITKIAG LTGDNLCKEGDKSFIANDTGEEDLHETQVGSLNDAYLVPLHEHCTLSQSAINDHNTKSHIAAEKSGPEKK LVYQEINLARKRQSVEAAIQVDPIEEETPKDLLPVLMLHQLQASVPGIHKTQNGVVQMPGSLAGVPFHSA ICNSSTNLLLAWLLVLNLKGSMNSFCQVDAHKATNKSSETLALLEILKHIAITEEADDLKAAVANLVEST TSHFGLSEKEQDMVPIDLSANCSTVNIQSVPKCSENERTQGISSLDGGCSASEACAPEVCVLEVTCSPCE MCTVNKAYSPKETCNPSDTFFPSDGYGVDQTSMNKACFLGEVCSLTDTVFSDKACAQKENHTYEGACPID ETYVPVNVCNTIDFLNSKENTYTDNLDSTEELERGDDIQKDLNILTDPEYKNGFNTLVSHQNVSNLSSCG LCLSEKEAELDKKHSSLDDFENCSLRKFQDENAYTSFDMEEPRTSEEPGSITNSMTSSERNISELESFEE LENHDTDIFNTVVNGGEQATEELIQEEVEASKTLELIDISSKNIMEEKRMNGIIYEIISKRLATPPSLDF CYDSKQNSEKETNEGETKMVKMMVKTMETGSYSESSPDLKKCIKSPVTSDWSDYRPDSDSEQPYKTSSDD PNDSGELTQEKEYNIGFVKRAIEKLYGKADIIKPSFFPGSTRKSQVCPYNSVEFQCSRKASLYDSEGQSF GSSEQVSSSSSMLQEFQEERQDKCDVSAVRDNYCRGDIVEPGTKQNDDSRILTDIEEGVLIDKGKWLLKE NHLLRMSSENPGMCGNADTTSVDTLLDNNSSEVPYSHFGNLAPGPTMDELSSSELEELTQPLELKCNYFN MPHGSDSEPFHEDLLDVRNETCAKERIANHHTEEKGSHQSERVCTSVTHSFISAGNKVYPVSDDAIKNQP LPGSNMIHGTLQEADSLDKLYALCGQHCPILTVIIQPMNEEDRGFAYRKESDIENFLGFYLWMKIHPYLL QTDKNVFREENNKASMRQNLIDNAIGDIFDQFYFSNTFDLMGKRRKQKRINFLGLEEEGNLKKFQPDLKE RFCMNFLHTSLLVVGNVDSNTQDLSGQTNEIFKAVDENNNLLNNRFQGSRTNLNQVVRENINCHYFFEML GQACLLDICQVETSLNISNRNILELCMFEGENLFIWEEEDILNLTDLESSREQEDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_006269
ORF Size	6468 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_006269.2
RefSeq Size	7100 bp
RefSeq ORF	6471 bp
Locus ID	6101
UniProt ID	P56715
Protein Families	Druggable Genome
MW	240.5 kDa
Gene Summary	This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. Transcript variants resulted from an alternative promoter and alternative splicings have been found, which overlap the current reference sequence and has several exons upstream and downstream of the current reference sequence. However, the biological validity and full-length nature of some variants cannot be determined at this time.[provided by RefSeq, Sep 2010]

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