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ASXL1 (NM_015338) Human Tagged ORF Clone
CAT＃: RC219354
ASXL1 (Myc-DDK-tagged)-Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1
ORF Plasmid: tGFP
"NM_015338" in other vectors (6)
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CNY 15,816.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BOPS; MDS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219354 representing NM_015338
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGAAGGACAAACAGAAGAAGAAGAAGGAGCGCACGTGGGCCGAGGCCGCGCGCCTGGTATTAGAAAACT ACTCGGATGCTCCAATGACACCAAAACAGATTCTGCAGGTCATAGAGGCAGAAGGACTAAAGGAAATGAG AAGTGGGACTTCCCCTCTCGCATGCCTCAATGCTATGCTACATTCCAATTCAAGAGGAGGAGAGGGGTTG TTTTATAAACTGCCTGGCCGAATCAGCCTTTTCACGCTCAAGAAGGATGCCCTGCAGTGGTCTCGCCATC CAGCTACAGTGGAGGGAGAGGAGCCAGAGGACACGGCTGATGTGGAGAGCTGTGGGTCTAATGAAGCCAG CACTGTGAGTGGTGAAAACGATGTATCTCTTGATGAAACATCTTCGAACGCATCCTGTTCTACAGAATCT CAGAGTCGACCTCTTTCCAATCCCAGGGACAGCTACAGAGCTTCCTCACAGGCGAACAAACAAAAGAAAA AGACTGGGGTGATGCTGCCTCGAGTTGTCCTGACTCCTCTGAAGGTAAACGGGGCCCACGTGGAATCTGC ATCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAGCCCGTCCAGCAGCAGCAGCGGCTCTCTG GCCCTGGGCAGCGCTGCTATTCGTGGCCAGGCCGAGGTCACCCAGGACCCTGCCCCGCTCCTGAGAGGCT TCCGGAAGCCAGCCACAGGTCAAATGAAGCGCAACAGAGGGGAAGAAATAGATTTTGAGACACCTGGGTC CATTCTTGTCAACACCAACCTCCGTGCCCTGATCAACTCTCGGACCTTCCATGCCTTACCATCACACTTC CAGCAGCAGCTCCTCTTCCTCCTGCCTGAAGTAGACAGACAGGTGGGGACGGATGGCCTGTTGCGTCTCA GCAGCAGTGCACTAAATAACGAGTTTTTTACCCATGCGGCTCAGAGCTGGCGGGAGCGCCTGGCTGATGG TGAATTTACTCATGAGATGCAAGTCAGGATACGACAGGAAATGGAGAAGGAAAAGAAGGTGGAACAATGG AAAGAAAAGTTCTTTGAAGACTACTATGGACAGAAGCTGGGTTTGACCAAAGAAGAGTCATTGCAGCAGA ACGTGGGCCAGGAGGAGGCTGAAATCAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACAGCG TGGTCCAGCCACCCGACAGCGAGATGGGCATTTTAAGAAACGCTCTCGGCCAGATCTCCGAACCAGAGCC AGAAGGAATCTGTACAAAAAACAGGAGTCAGAACAAGCAGGGGTTGCTAAGGATGCAAAATCTGTGGCCT CAGATGTTCCCCTCTACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCATCTGCC AGGCACATCCTCTGCAGCACCCGACCTGGAGGGTCCCGAATTCCCAGTTGAGTCTGTGGCTTCTCGGATC CAGGCTGAGCCAGACAACTTGGCACGTGCCTCTGCATCTCCAGACAGAATTCCTAGCCTGCCTCAGGAAA CTGTGGATCAGGAACCCAAGGATCAGAAGAGGAAATCCTTTGAGCAGGCGGCCTCTGCATCCTTTCCCGA AAAGAAGCCCCGGCTTGAAGATCGTCAGTCCTTTCGTAACACAATTGAAAGTGTTCACACCGAAAAGCCA CAGCCCACTAAAGAGGAGCCCAAAGTCCCGCCCATCCGGATTCAACTTTCACGTATCAAACCACCCTGGG TGGTTAAAGGTCAGCCCACTTACCAGATATGCCCCCGGATCATCCCCACCACGGAGTCCTCCTGCCGGGG TTGGACTGGCGCCAGGACCCTCGCAGACATTAAAGCCCGTGCTCTGCAGGTCCGAGGGGCGAGAGGTCAC CACTGCCATAGAGAGGCGGCCACCACTGCCATCGGAGGGGGGGGTGGCCCGGGTGGAGGTGGCGGCGGGG CCACCGATGAGGGAGGTGGCAGAGGCAGCAGCAGTGGTGATGGTGGTGAGGCCTGTGGCCACCCTGAGCC CAGGGGAGGCCCGAGCACCCCTGGAAAGTGTACGTCAGATCTACAGCGAACACAACTACTGCCGCCTTAT CCTCTAAATGGGGAGCATACCCAGGCCGGAACTGCCATGTCCAGAGCTAGGAGAGAGGACCTGCCTTCTC TGAGAAAGGAGGAAAGCTGCCTACTACAGAGGGCTACAGTTGGACTCACAGATGGGCTAGGAGATGCCTC CCAACTCCCCGTTGCTCCCACTGGGGACCAGCCATGCCAGGCCTTGCCCCTACTGTCCTCCCAAACCTCA GTAGCTGAGAGATTAGTGGAGCAGCCTCAGTTGCATCCGGATGTTAGAACTGAATGTGAGTCTGGCACCA CTTCCTGGGAAAGTGATGATGAGGAGCAAGGACCCACCGTTCCTGCAGACAATGGTCCCATTCCGTCTCT AGTGGGAGATGATACATTAGAGAAAGGAACTGGCCAAGCTCTTGACAGTCATCCCACTATGAAGGATCCT GTAAATGTGACCCCCAGTTCCACACCTGAATCCTCACCGACTGATTGCCTGCAGAACAGAGCATTTGATG ACGAATTAGGGCTTGGTGGCTCATGCCCTCCTATGAGGGAAAGTGATACTAGACAAGAAAACTTGAAAAC CAAGGCTCTCGTTTCTAACAGTTCTTTGCATTGGATACCCATCCCATCGAATGATGAGGTAGTGAAACAG CCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCCAGGTTGGAGAGGAGTGGGAGAAAGCTG CTCCCACCCCTCCTGCATTGCCTGGGGATTTGACAGCTGAGGAGGGTCTAGATCCTCTTGACAGCCTTAC TTCACTCTGGACTGTGCCATCTCGAGGAGGCAGTGACAGCAATGGCAGTTACTGTCAACAGGTGGACATT GAAAAGCTGAAAATCAACGGAGACTCTGAAGCACTGAGTCCTCACGGTGAGTCCACGGATACAGCCTCTG ACTTTGAAGGTCACCTCACGGAGGACAGCAGTGAGGCTGACACTAGAGAAGCTGCAGTGACAAAGGGATC TTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAATCTGCCCCACTGTCCAAGGTGAATGGTGACATG CGTCTGGTTACAAGGACAGATGGGATGGTTGCTCCTCAGAGCTGGGTGTCTCGAGTATGTGCGGTCCGCC AAAAGATCCCAGATTCCCTACTGCTGGCCAGTACTGAGTACCAGCCAAGAGCCGTGTGCCTGTCCATGCC TGGGTCCTCAGTGGAGGCCACTAACCCACTTGTGATGCAGTTGCTGCAGGGTAGCTTGCCCCTAGAGAAG GTTCTTCCACCAGCCCACGATGACAGCATGTCAGAATCCCCACAAGTACCACTTACAAAAGACCAGAGCC ATGGCTCGCTACGCATGGGATCTTTACATGGTCTTGGAAAAAACAGTGGCATGGTTGATGGAAGCAGCCC CAGTTCTTTAAGGGCTTTGAAGGAGCCTCTTCTGCCAGATAGCTGTGAAACAGGCACTGGTCTTGCCAGG ATTGAGGCCACCCAGGCTCCTGGAGCACCCCAAAAGAATTGCAAGGCAGTCCCAAGTTTTGACTCCCTCC ATCCAGTGACAAATCCCATTACATCCTCTAGGAAACTGGAAGAAATGGATTCCAAAGAGCAGTTCTCTTC CTTTAGTTGTGAAGATCAGAAGGAAGTCCGTGCTATGTCACAGGACAGTAATTCAAATGCTGCTCCAGGA AAGAGCCCAGGAGATCTTACTACCTCGAGAACACCTCGTTTCTCATCTCCAAATGTGATCTCCTTTGGTC CAGAGCAGACAGGTCGGGCCCTGGGTGATCAGAGCAATGTTACAGGCCAAGGGAAGAAGCTTTTTGGCTC TGGGAATGTGGCTGCAACCCTTCAGCGCCCCAGGCCTGCGGACCCGATGCCTCTTCCTGCTGAGATCCCT CCAGTTTTTCCCAGTGGGAAGTTGGGACCAAGCACAAACTCCATGTCTGGTGGGGTACAGACTCCAAGGG AAGACTGGGCTCCAAAGCCACATGCCTTTGTTGGCAGCGTCAAGAATGAGAAGACTTTTGTGGGGGGTCC TCTTAAGGCAAATGCCGAGAACAGGAAAGCTACTGGGCATAGTCCCCTGGAACTGGTGGGTCACTTGGAA GGGATGCCCTTTGTCATGGACTTGCCCTTCTGGAAATTACCCCGAGAGCCAGGGAAGGGGCTCAGTGAGC CTCTGGAGCCTTCTTCTCTCCCCTCCCAACTCAGCATCAAGCAGGCATTTTATGGGAAGCTTTCTAAACT CCAACTGAGTTCCACCAGCTTTAATTATTCCTCTAGCTCTCCCACCTTTCCCAAAGGCCTTGCTGGAAGT GTGGTGCAGCTGAGCCACAAAGCAAACTTTGGTGCGAGCCACAGTGCATCACTTTCCTTGCAAATGTTCA CTGACAGCAGCACGGTGGAAAGCATCTCGCTCCAGTGTGCGTGCAGCCTGAAAGCCATGATCATGTGCCA AGGCTGCGGTGCGTTCTGTCACGATGACTGTATTGGACCCTCAAAGCTCTGTGTATTGTGCCTTGTGGTG AGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219354 representing NM_015338
Red=Cloning site Green=Tags(s) MKDKQKKKKERTWAEAARLVLENYSDAPMTPKQILQVIEAEGLKEMRSGTSPLACLNAMLHSNSRGGEGL FYKLPGRISLFTLKKDALQWSRHPATVEGEEPEDTADVESCGSNEASTVSGENDVSLDETSSNASCSTES QSRPLSNPRDSYRASSQANKQKKKTGVMLPRVVLTPLKVNGAHVESASGFSGCHADGESGSPSSSSSGSL ALGSAAIRGQAEVTQDPAPLLRGFRKPATGQMKRNRGEEIDFETPGSILVNTNLRALINSRTFHALPSHF QQQLLFLLPEVDRQVGTDGLLRLSSSALNNEFFTHAAQSWRERLADGEFTHEMQVRIRQEMEKEKKVEQW KEKFFEDYYGQKLGLTKEESLQQNVGQEEAEIKSGLCVPGESVRIQRGPATRQRDGHFKKRSRPDLRTRA RRNLYKKQESEQAGVAKDAKSVASDVPLYKDGEAKTDPAGLSSPHLPGTSSAAPDLEGPEFPVESVASRI QAEPDNLARASASPDRIPSLPQETVDQEPKDQKRKSFEQAASASFPEKKPRLEDRQSFRNTIESVHTEKP QPTKEEPKVPPIRIQLSRIKPPWVVKGQPTYQICPRIIPTTESSCRGWTGARTLADIKARALQVRGARGH HCHREAATTAIGGGGGPGGGGGGATDEGGGRGSSSGDGGEACGHPEPRGGPSTPGKCTSDLQRTQLLPPY PLNGEHTQAGTAMSRARREDLPSLRKEESCLLQRATVGLTDGLGDASQLPVAPTGDQPCQALPLLSSQTS VAERLVEQPQLHPDVRTECESGTTSWESDDEEQGPTVPADNGPIPSLVGDDTLEKGTGQALDSHPTMKDP VNVTPSSTPESSPTDCLQNRAFDDELGLGGSCPPMRESDTRQENLKTKALVSNSSLHWIPIPSNDEVVKQ PKPESREHIPSVEPQVGEEWEKAAPTPPALPGDLTAEEGLDPLDSLTSLWTVPSRGGSDSNGSYCQQVDI EKLKINGDSEALSPHGESTDTASDFEGHLTEDSSEADTREAAVTKGSSVDKDEKPNWNQSAPLSKVNGDM RLVTRTDGMVAPQSWVSRVCAVRQKIPDSLLLASTEYQPRAVCLSMPGSSVEATNPLVMQLLQGSLPLEK VLPPAHDDSMSESPQVPLTKDQSHGSLRMGSLHGLGKNSGMVDGSSPSSLRALKEPLLPDSCETGTGLAR IEATQAPGAPQKNCKAVPSFDSLHPVTNPITSSRKLEEMDSKEQFSSFSCEDQKEVRAMSQDSNSNAAPG KSPGDLTTSRTPRFSSPNVISFGPEQTGRALGDQSNVTGQGKKLFGSGNVAATLQRPRPADPMPLPAEIP PVFPSGKLGPSTNSMSGGVQTPREDWAPKPHAFVGSVKNEKTFVGGPLKANAENRKATGHSPLELVGHLE GMPFVMDLPFWKLPREPGKGLSEPLEPSSLPSQLSIKQAFYGKLSKLQLSSTSFNYSSSSPTFPKGLAGS VVQLSHKANFGASHSASLSLQMFTDSSTVESISLQCACSLKAMIMCQGCGAFCHDDCIGPSKLCVLCLVV R TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015338 |
| ORF Size | 4623 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015338.6 |
| RefSeq Size | 7026 bp |
| RefSeq ORF | 4626 bp |
| Locus ID | 171023 |
| UniProt ID | Q8IXJ9 |
| MW | 165.3 kDa |
| Gene Summary | This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219354L1 | Lenti ORF clone of Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1, Myc-DDK-tagged |
CNY 18,216.00 |
|
| RC219354L2 | Lenti ORF clone of Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1, mGFP tagged |
CNY 17,290.00 |
|
| RC219354L3 | Lenti ORF clone of Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1, Myc-DDK-tagged |
CNY 18,216.00 |
|
| RC219354L4 | Lenti ORF clone of Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1, mGFP tagged |
CNY 18,216.00 |
|
| RG219354 | ASXL1 (tGFP-tagged) - Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1 |
CNY 17,416.00 |
|
| SC309230 | ASXL1 (untagged)-Human additional sex combs like 1 (Drosophila) (ASXL1), transcript variant 1 |
CNY 15,824.00 |
