PRB4 (NM_002723) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Po
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC219660 representing NM_002723 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGATTCTGCTGTCAGTGGCCCTGCTGGCCCTGAGCTCAGCTGAGAGTTCAAGTGAAGATGTCA GCCAGGAAGAATCTCTCTTCCTAATATCAGGAAAGCCAGAAGGACGACGCCCACAAGGAGGAAACCAGCC CCAACGTCCCCCACCTCCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGAGGAAACCAGTCCCAAGGT CCCCCACCTCCTCCAGGAAAGCCAGAAGGACGACCCCCACAAGGAGGCAACCAGTCCCAAGGTCCCCCAC CTCATCCAGGAAAGCCAGAAAGACCACCCCCACAAGGAGGAAACCAGTCCCAAGGTACCCCACCTCCTCC AGGAAAGCCAGAAAGACCACCCCCACAAGGAGGCAACCAGTCCCACCGTCCCCCACCTCCTCCAGGAAAG CCAGAAAGACCACCCCCACAAGGAGGTAACCAGTCCCAAGGTCCCCCACCTCATCCAGGAAAGCCAGAAG GACCACCCCCACAGGAAGGAAACAAGTCCCGAAGTGCCCGATCTCCTCCAGGAAAGCCACAAGGACCACC CCAACAAGAAGGCAACAAGCCTCAAGGTCCCCCACCTCCTGGAAAGCCACAAGGCCCACCCCCAGCAGGA GGCAATCCCCAGCAGCCTCAGGCACCTCCTGCTGGAAAGCCCCAGGGGCCACCTCCACCTCCTCAAGGGG GCAGGCCACCCAGACCTGCCCAGGGACAACAGCCTCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219660 representing NM_002723 Red=Cloning site Green=Tags(s) MLLILLSVALLALSSAESSSEDVSQEESLFLISGKPEGRRPQGGNQPQRPPPPPGKPQGPPPQGGNQSQG PPPPPGKPEGRPPQGGNQSQGPPPHPGKPERPPPQGGNQSQGTPPPPGKPERPPPQGGNQSHRPPPPPGK PERPPPQGGNQSQGPPPHPGKPEGPPPQEGNKSRSARSPPGKPQGPPQQEGNKPQGPPPPGKPQGPPPAG GNPQQPQAPPAGKPQGPPPPPQGGRPPRPAQGQQPPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_002723
ORF Size	741 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002723.6
RefSeq Size	944 bp
RefSeq ORF	744 bp
Locus ID	5545
Protein Families	Druggable Genome
MW	25.06 kDa
Gene Summary	This gene encodes a member of the heterogeneous family of basic, proline-rich, human salivary glycoproteins. The encoded preproprotein undergoes proteolytic processing to generate one or more mature peptides before secretion from the parotid glands. Multiple alleles of this gene exhibiting variations in the length of the tandem repeats have been identified. The reference genome encodes the "Small" allele. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015]

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