Collagen IV (COL4A3) (NM_000091) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ATS2; ATS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC223010 representing NM_000091 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGAGCGCCCGGACCGCCCCCAGGCCGCAGGTGCTCCTGCTGCCGCTCCTGCTGGTGCTCCTGGCGGCGG CGCCCGCAGCCAGCAAGGGTTGTGTCTGTAAAGACAAAGGCCAGTGCTTCTGTGACGGGGCCAAAGGGGA GAAGGGGGAGAAGGGCTTTCCTGGACCCCCCGGTTCTCCTGGCCAGAAAGGATTCACAGGTCCTGAAGGC TTGCCTGGACCGCAGGGACCCAAGGGCTTTCCAGGACTTCCAGGACTCACGGGTTCCAAAGGTGTAAGGG GAATAAGTGGATTGCCAGGATTTTCTGGTTCTCCTGGACTTCCAGGCACCCCAGGCAATACCGGGCCTTA CGGACTTGTCGGTGTACCAGGATGCAGTGGTTCTAAGGGTGAGCAGGGGTTTCCAGGACTCCCAGGGACA CTGGGCTACCCAGGGATCCCGGGTGCTGCTGGTTTGAAAGGACAAAAGGGTGCTCCTGCTAAAGAAGAAG ATATAGAACTTGATGCAAAAGGCGACCCCGGGTTGCCAGGGGCTCCAGGACCCCAGGGTTTGCCAGGCCC TCCAGGTTTTCCTGGGCCTGTTGGCCCACCTGGTCCTCCGGGATTCTTTGGCTTTCCAGGAGCCATGGGA CCTAGAGGACCTAAGGGTCACATGGGTGAAAGAGTGATAGGACATAAAGGAGAGCGGGGTGTGAAAGGGT TAACAGGACCCCCGGGACCACCAGGAACAGTTATTGTGACCCTAACTGGCCCAGATAACAGAACGGACCT CAAGGGGGAAAAGGGAGACAAGGGAGCAATGGGCGAGCCTGGACCTCCTGGACCCTCAGGACTGCCTGGA GAATCATATGGATCTGAAAAGGGTGCTCCTGGAGACCCTGGCCTGCAGGGAAAACCCGGAAAAGATGGTG TTCCTGGCTTCCCTGGAAGTGAGGGAGTCAAGGGCAACAGGGGTTTCCCTGGGTTAATGGGTGAAGATGG CATTAAGGGACAGAAAGGGGACATTGGCCCTCCAGGATTTCGTGGTCCAACAGAATATTATGACACATAC CAGGAAAAGGGAGATGAAGGCACTCCAGGCCCACCAGGGCCCAGAGGAGCTCGTGGCCCACAAGGTCCCA GTGGTCCCCCCGGAGTTCCTGGAAGTCCTGGATCATCAAGGCCTGGCCTCAGAGGAGCCCCTGGATGGCC AGGCCTGAAAGGAAGTAAAGGGGAACGAGGCCGCCCAGGAAAGGATGCCATGGGGACTCCTGGGTCCCCA GGTTGTGCTGGTTCACCAGGTCTTCCAGGATCACCGGGACCTCCAGGACCGCCAGGTGACATCGTTTTTC GCAAGGGTCCACCTGGAGATCACGGACTGCCAGGCTATCTAGGGTCTCCAGGAATCCCAGGAGTTGATGG GCCCAAAGGAGAACCAGGCCTCCTGTGTACACAGTGCCCTTATATCCCAGGGCCTCCCGGTCTCCCAGGA TTGCCAGGGTTACATGGTGTAAAAGGAATCCCAGGAAGACAAGGCGCAGCTGGCTTGAAAGGAAGCCCAG GGTCCCCAGGAAATACAGGTCTTCCAGGATTTCCAGGTTTCCCAGGTGCCCAGGGTGACCCAGGACTTAA AGGAGAAAAAGGTGAAACACTTCAGCCTGAGGGGCAAGTGGGTGTCCCAGGTGACCCGGGGCTCAGAGGC CAACCTGGGAGAAAGGGCTTGGATGGAATTCCTGGAACTCCGGGAGTGAAAGGATTACCAGGACCTAAAG GCGAACTGGCTCTGAGTGGTGAGAAAGGGGACCAAGGTCCTCCAGGGGATCCTGGCTCCCCTGGGTCCCC AGGACCTGCAGGACCAGCTGGACCACCTGGCTACGGACCCCAAGGAGAACCTGGTCTCCAGGGCACGCAA GGAGTTCCTGGAGCCCCCGGACCACCCGGAGAAGCCGGCCCTAGGGGAGAGCTCAGTGTTTCAACACCAG TTCCAGGCCCACCAGGACCTCCAGGGCCCCCTGGCCATCCTGGCCCCCAAGGTCCACCTGGTATCCCTGG ATCCCTGGGGAAATGTGGAGATCCTGGTCTTCCAGGGCCTGATGGTGAACCAGGAATTCCAGGAATTGGA TTTCCTGGGCCTCCTGGACCTAAGGGAGACCAAGGTTTTCCAGGTACAAAAGGATCACTGGGTTGTCCTG GAAAAATGGGAGAGCCTGGGTTACCTGGAAAGCCAGGCCTCCCAGGAGCCAAGGGAGAACCAGCAGTAGC CATGCCTGGAGGACCAGGAACACCAGGTTTTCCAGGAGAAAGAGGCAATTCTGGGGAACATGGAGAAATT GGACTCCCTGGACTTCCAGGTCTCCCTGGAACTCCAGGAAATGAAGGGCTTGATGGACCACGAGGAGATC CAGGGCAGCCTGGACCACCTGGAGAACAAGGACCCCCAGGAAGGTGCATAGAGGGTCCCAGGGGAGCCCA AGGACTTCCAGGCTTAAATGGATTGAAAGGGCAACAAGGCAGAAGAGGTAAAACGGGGCCAAAGGGAGAC CCAGGAATTCCAGGCTTGGATAGATCAGGATTTCCTGGAGAAACTGGATCACCAGGAATTCCAGGTCATC AAGGTGAAATGGGACCACTGGGTCAAAGAGGATATCCAGGAAATCCGGGAATTTTAGGGCCACCAGGTGA AGATGGAGTGATTGGGATGATGGGCTTTCCTGGAGCCATTGGCCCTCCAGGGCCCCCTGGGAACCCAGGC ACACCAGGGCAGAGGGGGAGCCCTGGAATTCCAGGAGTAAAGGGCCAGAGAGGAACCCCAGGAGCCAAGG GGGAACAAGGAGATAAAGGAAATCCCGGGCCTTCAGAGATATCCCACGTAATAGGGGACAAAGGAGAACC AGGTCTCAAAGGATTCGCAGGAAATCCAGGTGAGAAAGGAAACAGAGGCGTTCCAGGGATGCCAGGTTTA AAGGGCCTCAAAGGACTACCCGGACCAGCAGGACCACCAGGCCCCAGAGGAGATTTGGGCAGCACTGGGA ATCCTGGAGAACCAGGACTGCGTGGTATACCAGGAAGCATGGGGAACATGGGCATGCCAGGTTCTAAAGG AAAAAGGGGAACTTTGGGATTCCCAGGTCGAGCAGGAAGACCAGGCCTCCCAGGTATTCATGGTCTCCAG GGAGATAAGGGAGAGCCAGGTTATTCAGAAGGTACAAGGCCAGGACCACCGGGACCAACGGGGGATCCAG GACTGCCGGGTGATATGGGAAAGAAAGGAGAAATGGGGCAACCTGGCCCACCTGGACATTTGGGGCCTGC TGGACCTGAGGGAGCCCCTGGAAGTCCTGGAAGTCCTGGCCTCCCAGGAAAGCCAGGTCCTCATGGTGAT TTGGGTTTTAAAGGAATCAAAGGCCTCCTGGGCCCTCCAGGAATCAGAGGCCCTCCAGGTCTTCCAGGAT TTCCAGGATCTCCTGGACCAATGGGTATAAGAGGTGACCAAGGACGTGATGGAATTCCTGGTCCAGCCGG AGAAAAGGGAGAAACGGGTTTATTGAGGGCCCCTCCAGGCCCAAGAGGGAACCCTGGTGCTCAAGGAGCC AAAGGAGACAGGGGAGCCCCAGGTTTTCCTGGCCTCCCGGGCAGAAAAGGGGCCATGGGAGATGCTGGAC CTCGAGGACCCACAGGCATAGAAGGATTCCCAGGGCCACCAGGTCTGCCCGGTGCAATTATCCCTGGCCA GACAGGAAATCGTGGTCCACCAGGCTCAAGAGGAAGCCCAGGTGCGCCTGGTCCCCCTGGACCTCCAGGG AGTCATGTAATAGGCATAAAAGGAGACAAAGGGTCTATGGGCCACCCTGGCCCAAAAGGTCCACCTGGAA CTGCAGGAGACATGGGACCACCAGGTCGTCTGGGAGCACCAGGTACTCCAGGTCTTCCAGGACCCAGAGG TGATCCTGGATTCCAGGGGTTTCCAGGCGTGAAAGGAGAAAAGGGTAATCCTGGATTTCTAGGATCCATT GGACCTCCAGGACCAATTGGGCCAAAAGGACCACCTGGTGTACGTGGAGACCCTGGCACACTTAAGATTA TCTCCCTTCCAGGAAGCCCAGGGCCACCTGGCACACCTGGAGAACCAGGGATGCAGGGAGAACCTGGGCC ACCAGGGCCACCTGGAAACCTAGGACCCTGTGGGCCAAGAGGTAAGCCAGGCAAGGATGGAAAACCAGGA ACTCCTGGACCAGCTGGAGAAAAAGGCAACAAAGGTTCTAAAGGAGAGCCAGGACCAGCTGGATCAGATG GATTGCCAGGTTTGAAAGGAAAACGTGGAGACAGTGGATCACCTGCAACCTGGACAACGAGAGGCTTTGT CTTCACCCGACACAGTCAAACCACAGCAATTCCTTCATGTCCAGAGGGGACAGTGCCACTCTACAGTGGG TTTTCTTTTCTTTTTGTACAAGGAAATCAACGAGCCCACGGACAAGACCTTGGAACTCTTGGCAGCTGCC TGCAGCGATTTACCACAATGCCATTCTTATTCTGCAATGTCAATGATGTATGTAATTTTGCATCTCGAAA TGATTATTCATACTGGCTGTCAACACCAGCTCTGATGCCAATGAACATGGCTCCCATTACTGGCAGAGCC CTTGAGCCTTATATAAGCAGATGCACTGTTTGTGAAGGTCCTGCGATCGCCATAGCCGTTCACAGCCAAA CCACTGACATTCCTCCATGTCCTCACGGCTGGATTTCTCTCTGGAAAGGATTTTCATTCATCATGTTCAC AAGTGCAGGTTCTGAGGGCACCGGGCAAGCACTGGCCTCCCCTGGCTCCTGCCTGGAAGAATTCCGAGCC AGCCCATTTCTAGAATGTCATGGAAGAGGAACGTGCAACTACTATTCAAATTCCTACAGTTTCTGGCTGG CTTCATTAAACCCAGAAAGAATGTTCAGAAAGCCTATTCCATCAACTGTGAAAGCTGGGGAATTAGAAAA AATAATAAGTCGCTGTCAGGTGTGCATGAAGAAAAGACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC223010 representing NM_000091 Red=Cloning site Green=Tags(s) MSARTAPRPQVLLLPLLLVLLAAAPAASKGCVCKDKGQCFCDGAKGEKGEKGFPGPPGSPGQKGFTGPEG LPGPQGPKGFPGLPGLTGSKGVRGISGLPGFSGSPGLPGTPGNTGPYGLVGVPGCSGSKGEQGFPGLPGT LGYPGIPGAAGLKGQKGAPAKEEDIELDAKGDPGLPGAPGPQGLPGPPGFPGPVGPPGPPGFFGFPGAMG PRGPKGHMGERVIGHKGERGVKGLTGPPGPPGTVIVTLTGPDNRTDLKGEKGDKGAMGEPGPPGPSGLPG ESYGSEKGAPGDPGLQGKPGKDGVPGFPGSEGVKGNRGFPGLMGEDGIKGQKGDIGPPGFRGPTEYYDTY QEKGDEGTPGPPGPRGARGPQGPSGPPGVPGSPGSSRPGLRGAPGWPGLKGSKGERGRPGKDAMGTPGSP GCAGSPGLPGSPGPPGPPGDIVFRKGPPGDHGLPGYLGSPGIPGVDGPKGEPGLLCTQCPYIPGPPGLPG LPGLHGVKGIPGRQGAAGLKGSPGSPGNTGLPGFPGFPGAQGDPGLKGEKGETLQPEGQVGVPGDPGLRG QPGRKGLDGIPGTPGVKGLPGPKGELALSGEKGDQGPPGDPGSPGSPGPAGPAGPPGYGPQGEPGLQGTQ GVPGAPGPPGEAGPRGELSVSTPVPGPPGPPGPPGHPGPQGPPGIPGSLGKCGDPGLPGPDGEPGIPGIG FPGPPGPKGDQGFPGTKGSLGCPGKMGEPGLPGKPGLPGAKGEPAVAMPGGPGTPGFPGERGNSGEHGEI GLPGLPGLPGTPGNEGLDGPRGDPGQPGPPGEQGPPGRCIEGPRGAQGLPGLNGLKGQQGRRGKTGPKGD PGIPGLDRSGFPGETGSPGIPGHQGEMGPLGQRGYPGNPGILGPPGEDGVIGMMGFPGAIGPPGPPGNPG TPGQRGSPGIPGVKGQRGTPGAKGEQGDKGNPGPSEISHVIGDKGEPGLKGFAGNPGEKGNRGVPGMPGL KGLKGLPGPAGPPGPRGDLGSTGNPGEPGLRGIPGSMGNMGMPGSKGKRGTLGFPGRAGRPGLPGIHGLQ GDKGEPGYSEGTRPGPPGPTGDPGLPGDMGKKGEMGQPGPPGHLGPAGPEGAPGSPGSPGLPGKPGPHGD LGFKGIKGLLGPPGIRGPPGLPGFPGSPGPMGIRGDQGRDGIPGPAGEKGETGLLRAPPGPRGNPGAQGA KGDRGAPGFPGLPGRKGAMGDAGPRGPTGIEGFPGPPGLPGAIIPGQTGNRGPPGSRGSPGAPGPPGPPG SHVIGIKGDKGSMGHPGPKGPPGTAGDMGPPGRLGAPGTPGLPGPRGDPGFQGFPGVKGEKGNPGFLGSI GPPGPIGPKGPPGVRGDPGTLKIISLPGSPGPPGTPGEPGMQGEPGPPGPPGNLGPCGPRGKPGKDGKPG TPGPAGEKGNKGSKGEPGPAGSDGLPGLKGKRGDSGSPATWTTRGFVFTRHSQTTAIPSCPEGTVPLYSG FSFLFVQGNQRAHGQDLGTLGSCLQRFTTMPFLFCNVNDVCNFASRNDYSYWLSTPALMPMNMAPITGRA LEPYISRCTVCEGPAIAIAVHSQTTDIPPCPHGWISLWKGFSFIMFTSAGSEGTGQALASPGSCLEEFRA SPFLECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGELEKIISRCQVCMKKRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	AscI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_000091
ORF Size	5010 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000091.5
RefSeq Size	8050 bp
RefSeq ORF	5013 bp
Locus ID	1285
UniProt ID	Q01955
Protein Families	Druggable Genome
MW	161.81 kDa
Gene Summary	Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]

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