C2 (NM_001282457) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARMD14; CO2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238614 representing NM_001282457 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACAAGGTCCGCTATCGCTGCTCCTCGAATCTTGTGCTCACGGGGTCTTCGGAGCGGGAGTGCCA GGGCAACGGGGTCTGGAGTGGAACGGAGCCCATCTGCCGCCATCTTCAGCTTTGAGATCAATGTGAGCGT TGCCATTATCACCTTTGCCTCAGAGCCCAAAGTCCTCATGTCTGTCCTGAACGACAACTCCCGGGATATG ACTGAGGTGATCAGCAGCCTGGAAAATGCCAACTATAAAGATCATGAAAATGGAACTGGGACTAACACCT ATGCGGCCTTAAACAGTGTCTATCTCATGATGAACAACCAAATGCGACTCCTCGGCATGGAAACGATGGC CTGGCAGGAAATCCGACATGCCATCATCCTTCTGACAGATGGAAAGTCCAATATGGGTGGCTCTCCCAAG ACAGCTGTTGACCATATCAGAGAGATCCTGAACATCAACCAGAAGAGGAATGACTATCTGGACATCTATG CCATCGGGGTGGGCAAGCTGGATGTGGACTGGAGAGAACTGAATGAGCTAGGGTCCAAGAAGGATGGTGA GAGGCATGCCTTCATTCTGCAGGACACAAAGGCTCTGCACCAGGTCTTTGAACATATGCTGGATGTCTCC AAGCTCACAGACACCATCTGCGGGGTGGGGAACATGTCAGCAAACGCCTCTGACCAGGAGAGGACACCCT GGCATGTCACTATTAAGCCCAAGAGCCAAGAGACCTGCCGGGGGGCCCTCATCTCCGACCAATGGGTCCT GACAGCAGCTCATTGCTTCCGCGATGGCAACGACCACTCCCTGTGGAGGGTCAATGTGGGAGACCCCAAA TCCCAGTGGGGCAAAGAATTCCTTATTGAGAAGGCGGTGATCTCCCCAGGGTTTGATGTCTTTGCCAAAA AGAACCAGGGAATCCTGGAGTTCTATGGTGATGACATAGCTCTGCTGAAGCTGGCCCAGAAAGTAAAGAT GTCCACCCATGCCAGGCCCATCTGCCTTCCCTGCACGATGGAGGCCAATCTGGCTCTGCGGAGACCTCAA GGCAGCACCTGTAGGGACCATGAGAATGAACTGCTGAACAAACAGAGTGTTCCTGCTCATTTTGTCGCCT TGAATGGGAGCAAACTGAACATTAACCTTAAGATGGGAGTGGAGTGGACAAGCTGTGCCGAGGTTGTCTC CCAAGAAAAAACCATGTTCCCCAACTTGACAGATGTCAGGGAGGTGGTGACAGACCAGTTCCTATGCAGT GGGACCCAGGAGGATGAGAGTCCCTGCAAGGGAGAATCTGGGGGAGCAGTTTTCCTTGAGCGGAGATTCA GGTTTTTTCAGGTGGGTCTGGTGAGCTGGGGTCTTTACAACCCCTGCCTTGGCTCTGCTGACAAAAACTC CCGCAAAAGGGCCCCTCGTAGCAAGGTCCCGCCGCCACGAGACTTTCACATCAATCTCTTCCGCATGCAG CCCTGGCTGAGGCAGCACCTGGGGGATGTCCTGAATTTTTTACCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238614 representing NM_001282457 Red=Cloning site Green=Tags(s) MGTRSAIAAPRILCSRGLRSGSARATGSGVERSPSAAIFSFEINVSVAIITFASEPKVLMSVLNDNSRDM TEVISSLENANYKDHENGTGTNTYAALNSVYLMMNNQMRLLGMETMAWQEIRHAIILLTDGKSNMGGSPK TAVDHIREILNINQKRNDYLDIYAIGVGKLDVDWRELNELGSKKDGERHAFILQDTKALHQVFEHMLDVS KLTDTICGVGNMSANASDQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRDGNDHSLWRVNVGDPK SQWGKEFLIEKAVISPGFDVFAKKNQGILEFYGDDIALLKLAQKVKMSTHARPICLPCTMEANLALRRPQ GSTCRDHENELLNKQSVPAHFVALNGSKLNINLKMGVEWTSCAEVVSQEKTMFPNLTDVREVVTDQFLCS GTQEDESPCKGESGGAVFLERRFRFFQVGLVSWGLYNPCLGSADKNSRKRAPRSKVPPPRDFHINLFRMQ PWLRQHLGDVLNFLPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282457
ORF Size	1518 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282457.2
RefSeq Size	2069 bp
RefSeq ORF	1521 bp
Locus ID	717
Protein Families	Druggable Genome, Protease, Secreted Protein
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus
MW	56.9 kDa
Gene Summary	Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]

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