MAN2B2 (NM_001292038) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239824 representing NM_001292038 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGCTGTGCTGGCTGCCGCTGCTGGCACCGCTCCTGTTGCTGCGACCGCCAGGGGTCCAGTCCG CCGGCCCCATCCGGGCCTTCGTGGTGCCCCACAGCCACATGGACGTGGGCTGGGTCTACACTGTGCAGGA AAGCATGCGGGCGTACGCCGCCAATGTCTACACCTCAGTGGTGGAAGAGCTGGCCCGCGGCCAGCAGCGC CGGTTCATCGCTGTGGAGCAGGAGTTTTTCCGGCTGTGGTGGGATGGCGTCGCCTCGGACCAGCAGAAAT ACCAGGTCCGCCAGCTCCTGGAGGAAGGACGCCTGGAATTTGTCATCGGAGGCCAGGTCATGCATGACGA GGCTGTGACGCACCTTGATGACCAGATCCTGCAGCTCACAGAAGGACACGGGTTTCTCTATGAAACATTT GGGATCCGGCCACAGTTCTCCTGGCACGTTGACCCGTTTGGCGCCTCTGCCACGACGCCCACCCTATTTG CGCTGGCGGGCTTCAATGCCCACCTCGGCTCCCGGATCGACTACGACCTGAAGGCAGCCATGCAGGAGGC CCGGGGGCTGCAGTTCGTGTGGCGAGGGTCCCCATCCCTCTCAGAGCGGCAGGAAATCTTCACGCACATC ATGGACCAGTACAGCTACTGCACCCCGTCCCACATCCCTTTCTCCAACAGGTCAGGATTTTACTGGAATG GCGTGGCTGTCTTCCCCAAGCCTCCCCAAGATGGGGTGTACCCCAACATGAGTGAGCCTGTCACCCCAGC CAACATCAACCTCTATGCCGAGCTCGGTGTCTCGGTGCAGTATGCCACGCTGGGCGACTACTTCCGTGCC CTGCACGCTCTCAATGTCACCTGGCGTGTCCGCGACCACCACGACTTCCTGCCCTATTCCACAGAACCAT TCCAGGCCTGGACGGGCTTCTACACGTCCCGCAGCTCACTGAAGGGGCTGGCCCGGCGAGCCAGCGCCTT GTTGTATGCCGGGGAGTCCATGTTCACACGCTACCTGTGGCCGGCCCCCCGTGGGCATCTGGACCCCACC TGGGCCCTGCAGCAGCTCCAGCAGCTTCGCTGGGCCGTCTCCGAGGTCCAGCACCATGATGCCATCACTG GGACTGAGTCCCCCAAGGTGAGAGACATGTACGCAACGCACCTGGCCTCGGGGATGCTGGGCATGCGCAA GCTGATGGCCTCCATCGTCCTAGATGAGCTCCAGCCCCAGGCACCCATGGCGGCCAGCTCCGATGCAGGA CCTGCAGGACATTTTGCCTCGGTCTACAACCCGCTGGCCTGGACGGTCACCACCATCGTCACCCTGACTG TTGGTTTCCCTGGAGTCCGCGTCACAGATGAGGCGGGCCACCCAGTGCCCTCGCAGATCCAGAACTCAAC AGAGACCCCATCTGCGTATGACCTGCTTATTCTGACCACAATCCCAGGCCTCAGTTACCGGCACTACAAC ATCAGACCCACTGCAGGGGCCCAAGAGGGCACCCAGGAGCCGGCTGCCACTGTGGCGAGCACCCTTCAAT TTGGCCGCAGGCTGAGGAGACGCACCAGCCATGCGGGCAGGTACTTGGTGCCTGTGGCAAACGACTGCTA CATTGTGCTGCTCGACCAGGATACCAACCTGATGCACAGCATCTGGGAGAGACAGAGTAACCGAACGGTG CGCGTGACCCAGGAATTCCTGGAGTACCACGTCAACGGGGATGTGAAACAGGGCCCCATTTCCGATAACT ACCTGTTCACACCGGGCAAGGCCGCGGTGCCTGCGTGGGAAGCTGTGGAAATGGAGATTGTGGCGGGACA GCTTGTGACTGAGATCCGGCAGTACTTCTACAGGAACATGACAGCACAGAATTACACGTATGCAATCCGC TCCCGGCTCACCCATGTGCCGCAGGGCCATGACGGGGAGCTGCTCTGCCACCGGATAGAGCAGGAGTACC AAGCCGGCCCCCTGGAGCTGAACCGTGAGGCTGTCCTGAGGACCAGCACCAACCTAAACAGCCAGCAGGT CATCTACTCAGACAACAACGGCTACCAGATGCAGCGGAGGCCCTACGTTTCCTATGTGAACAACAGCATC GCCCGGAATTACTACCCCATGGTTCAGTCGGCCTTCATGGAGGATGGCAAAAGCAGGCTTGTGTTGCTGT CGGAGCGGGCACATGGCATCTCCAGCCAAGGGAATGGGCAGGTGGAGGTCATGCTCCACCGGCGGCTGTG GAACAACTTCGACTGGGACCTGGGCTACAACCTCACGCTGAACGACACCTCAGTCGTCCACCCAGTGCTC TGGCTTCTGCTGGGATCCTGGTCCCTCACCACTGCCCTGCGCCAGAGGAGCGCACTGGCGCTGCAGCACA GGCCCGTGGTGCTGTTCGGAGACCTCGCTGGGACTGCGCCGAAGCTCCCAGGACCCCAGCAGCAAGAGGC CGTGACGCTGCCCCCGAATCTTCACCTGCAGATCCTGAGCATCCCTGGCTGGCGCTACAGCTCCAACCAC ACGGAGCACTCTCAGAATCTCCGGAAAGGCCATCGAGGGGAAGCCCAGGCTGACCTCCGCCGTGTCCTGC TGCGGCTCTACCACCTGTATGAAGTGGGCGAGGACCCAGTCCTGTCTCAGCCAGTGACAGTGAATCTGGA GGCTGTGCTGCAGGCGCTGGGGTCCGTGGTGGCAGTGGAGGAGCGCTCGCTCACAGGGACCTGGGATTTG AGCATGCTGCACCGCTGGAGCTGGAGGACGGGGCCTGGCCGCCACAGAGGTGACACCACCTCTCCCTCGA GGCCACCAGGAGGCCCCATCATCACCGTCCACCCAAAGGAAATCCGGACGTTCTTTATTCACTTTCAACA GCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239824 representing NM_001292038 Red=Cloning site Green=Tags(s) MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQR RFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETF GIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHI MDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPQDGVYPNMSEPVTPANINLYAELGVSVQYATLGDYFRA LHALNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPT WALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGMRKLMASIVLDELQPQAPMAASSDAG PAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYN IRPTAGAQEGTQEPAATVASTLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTV RVTQEFLEYHVNGDVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIR SRLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSI ARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVL WLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNH TEHSQNLRKGHRGEAQADLRRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDL SMLHRWSWRTGPGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001292038
ORF Size	2874 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001292038.1, NP_001278967.1
RefSeq Size	4048 bp
RefSeq ORF	2877 bp
Locus ID	23324
UniProt ID	Q9Y2E5
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Other glycan degradation
MW	108.6 kDa

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