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hHR23A (RAD23A) (NM_001270362) Human Tagged ORF Clone
CAT＃: RG232582
- TrueORF®
RAD23A (tGFP-tagged) - Homo sapiens RAD23 homolog A (S. cerevisiae) (RAD23A), transcript variant 2
ORF Plasmid: DDK
"NM_001270362" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HHR23A; HR23A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232582 representing NM_001270362
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTCACCATCACGCTCAAAACGCTGCAGCAGCAGACCTTCAAGATCCGCATGGAGCCTGACGAGA CGGTGAAGGTGCTAAAGGAGAAGATAGAAGCTGAGAAGGGTCGTGATGCCTTCCCCGTGGCTGGACAGAA ACTCATCTATGCCGGCAAGATCTTGAGTGACGATGTCCCTATCAGGGACTATCGCATCGATGAGAAGAAC TTTGTGGTCGTCATGGTGACCAAGACCAAAGCCGGCCAGGGTACCTCAGCACCCCCAGAGGCCTCACCCA CAGCTGCCCCAGAGTCCTCTACATCCTTCCCGCCTGCCCCCACCTCAGGCATGTCCCATCCCCCACCTGC CGCCAGAGAGGACAAGAGCCCATCAGAGGAATCCGCCCCCACGACGTCCCCAGAGTCTGTGTCAGGCTCT GTTCCCTCTTCAGGTAGCAGCGGGCGAGAGGAAGACGCGGCCTCCACGCTAGTGACGGGCTCTGAGTATG AGACGATGCTGACGGAGATCATGTCCATGGGCTATGAGCGAGAGCGGGTCGTGGCCGCCCTGAGAGCCAG CTACAACAACCCCCACCGAGCCGTGGAGTATCTGCTCACGGGAATTCCTGGGAGCCCCGAGCCGGAACAC GGTTCTGTCCAGGAGAGCCAGGTATCGGAGCAGCCGGCCACGGAAGCAGGAGAGAACCCCCTGGAGTTCC TGCGGGACCAGCCCCAGTTCCAGAACATGCGGCAGGTGATTCAGCAGAACCCTGCGCTGCTGCCCGCCCT GCTCCAGCAGCTGGGCCAGGAGAACCCTCAGCTTTTACAGCAAATCAGCCGGCACCAGGAGCAGTTCATC CAGATGCTGAACGAGCCCCCTGGGGAGCTGGCGGACATCTCAGATGTGGAGGGGGAGGTGGGCGCCATAG GAGAGGAGGCCCCGCAGATGAACTACATCCAGGTGACGCCGCAGGAGAAAGAAGCTATAGAGAGGTTGAA GGCCCTGGGCTTCCCAGAGAGCCTGGTCATCCAGGCCTATTTCGCGTGTGAAAAAAATGAGAACTTGGCT GCCAACTTCCTCCTGAGTCAGAACTTTGATGACGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232582 representing NM_001270362
Red=Cloning site Green=Tags(s) MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKN FVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGS VPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEH GSVQESQVSEQPATEAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFI QMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLA ANFLLSQNFDDE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001270362 |
| ORF Size | 1086 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001270362.1, NP_001257291.1 |
| RefSeq Size | 1818 bp |
| RefSeq ORF | 1089 bp |
| Locus ID | 5886 |
| UniProt ID | P54725 |
| Protein Families | Druggable Genome |
| Protein Pathways | Nucleotide excision repair |
| Gene Summary | The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012] |
Documents
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