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CLCN6 (NM_001256959) Human Tagged ORF Clone
CAT＃: RG234960
- TrueORF®
CLCN6 (tGFP-tagged) - Homo sapiens chloride channel, voltage-sensitive 6 (CLCN6), transcript variant 2
ORF Plasmid: DDK
"NM_001256959" in other vectors (2)
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CNY 7,890.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CLC-6; CONRIBA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234960 representing NM_001256959
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCC GCACCCCCGAGGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGA CTATGAGAAAGGTCGAAGATATGAGGCGGTGAAGTGGATGGTGGTGTTTGCCATTGGAGTCTGCACTGGC CTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCAACTCAAGTTCGGAGTGGTACAGACAT CGGTGGAGGAGTGCAGCCAGAAAGGCTGCCTCGCTCTGTCTCTCCTTGAACTCCTGGGTTTTAACCTCAC CTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGGATACCCGAGGTC AAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTG GAGTGCTGTTCAGTGTGGCTGGAGGGCTCTTCGTGGGGAAGGAAGGCCCCATGATCCACAGTGGTTCGGT GGTGGGAGCTGGCCTCCCTCAGTTTCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTCCCCTATTTC CGAAGCGACAGAGACAAGAGAGACTTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGG CGCCAATCGGGGGTACCTTGTTCAGTCTAGAGGAGGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAA AGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCAACTTCTTCCGTTCTGGGATTCAGTTTGGAAGC TGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGTGCTCTGACTCTGATAAAAAAT GTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGGGGCCTCCTGGGAGC CACATTCAACTGTCTGAACAAGAGGCTTGCAAAGTACCGTATGCGAAACGTGCACCCGAAACCTAAGCTC GTCAGAGTCTTAGAGAGCCTCCTTGTGTCTCTGGTAACCACCGTGGTGGTGTTTGTGGCCTCGATGGTGT TAGGAGAATGCCGACAGATGTCCTCTTCGAGTCAAATCGGTAATGACTCATTCCAGCTCCAGGTCACAGA AGATGTGAATTCAAGTATCAAGACATTTTTTTGTCCCAATGATACCTACAATGACATGGCCACACTCTTC TTCAACCCGCAGGAGTCTGCCATCCTCCAGCTCTTCCACCAGGATGGTACTTTCAGCCCCGTCACTCTGG CCTTGTTCTTCGTTCTCTATTTCTTGCTTGCATGTTGGACTTACGGCATTTCTGTTCCAAGTGGCCTTTT TGTGCCTTCTCTGCTGTGTGGAGCTGCTTTTGGACGTTTAGTTGCCAATGTCCTAAAAAGCTACATTGGA TTGGGCCACATCTATTCGGGGACCTTTGCCCTGATTGGTGCAGCGGCTTTCTTGGGCGGGGTGGTCCGCA TGACCATCAGCCTCACGGTCATCCTGATCGAGTCCACCAATGAGATCACCTACGGGCTCCCCATCATGGT CACACTGATGGTGGCCAAATGGACAGGGGACTTTTTCAATAAGGGCATTTATGATATCCACGTGGGCCTG CGAGGCGTGCCGCTTCTGGAATGGGAGACAGAGGTGGAAATGGACAAGCTGAGAGCCAGCGACATCATGG AGCCCAACCTGACCTACGTCTACCCGCACACCCGCATCCAGTCTCTGGTGAGCATCCTGCGCACCACGGT CCACCATGCCTTCCCGGTGGTCACAGAGAACCGCGGTAACGAGAAGGAGTTCATGAAGGGCAACCAGCTC ATCAGCAACAACATCAAGTTCAAGAAATCCAGCATCCTCACCCGGGCTGGCGAGCAGCGCAAACGGAGCC AGTCCATGAAGTCCTACCCATCCAGCGAGCTACGGAACATGTGTGATGAGCACATCGCCTCTGAGGAGCC AGCCGAGAAGGAGGACCTCCTGCAGCAGATGCTGGAAAGGAGATACACTCCCTACCCCAACCTATACCCT GACCAGTCCCCAAGTGAAGACTGGACCATGGAGGAGCGGTTCCGCCCTCTGACCTTCCACGGCCTGATCC TTCGGTCGCAGCTTGTCACCCTGCTTGTCCGAGGAGTTTGTTACTCTGAAAGCCAGTCGAGCGCCAGCCA GCCGCGCCTCTCCTATGCCGAGATGGCCGAGGACTACCCGCGGTACCCCGACATCCACGACCTGGACCTG ACGCTGCTCAACCCGCGCATGATCGTGGATGTCACCCCATACATGAACCCTTCGCCTTTCACCGTCTCGC CCAACACCCACGTCTCCCAAGTCTTCAACCTGTTCAGAACGATGGGCCTGCGCCACCTGCCCGTGGTGAA CGCTGTGGGAGAGATCGTGGGGATCATCACACGGCACAACCTCACCTATGAATTTCTGCAGGCCCGGCTG AGGCAGCACTACCAGACCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234960 representing NM_001256959
Red=Cloning site Green=Tags(s) MAGCRGSLCCCCRWCCCCGERETRTPEELTILGETQEEEDEILPRKDYEKGRRYEAVKWMVVFAIGVCTG LVGLFVDFFVRLFTQLKFGVVQTSVEECSQKGCLALSLLELLGFNLTFVFLASLLVLIEPVAAGSGIPEV KCYLNGVKVPGIVRLRTLLCKVLGVLFSVAGGLFVGKEGPMIHSGSVVGAGLPQFQSISLRKIQFNFPYF RSDRDKRDFVSAGAAAGVAAAFGAPIGGTLFSLEEGSSFWNQGLTWKVLFCSMSATFTLNFFRSGIQFGS WGSFQLPGLLNFGEFKCSDSDKKCHLWTAMDLGFFVVMGVIGGLLGATFNCLNKRLAKYRMRNVHPKPKL VRVLESLLVSLVTTVVVFVASMVLGECRQMSSSSQIGNDSFQLQVTEDVNSSIKTFFCPNDTYNDMATLF FNPQESAILQLFHQDGTFSPVTLALFFVLYFLLACWTYGISVPSGLFVPSLLCGAAFGRLVANVLKSYIG LGHIYSGTFALIGAAAFLGGVVRMTISLTVILIESTNEITYGLPIMVTLMVAKWTGDFFNKGIYDIHVGL RGVPLLEWETEVEMDKLRASDIMEPNLTYVYPHTRIQSLVSILRTTVHHAFPVVTENRGNEKEFMKGNQL ISNNIKFKKSSILTRAGEQRKRSQSMKSYPSSELRNMCDEHIASEEPAEKEDLLQQMLERRYTPYPNLYP DQSPSEDWTMEERFRPLTFHGLILRSQLVTLLVRGVCYSESQSSASQPRLSYAEMAEDYPRYPDIHDLDL TLLNPRMIVDVTPYMNPSPFTVSPNTHVSQVFNLFRTMGLRHLPVVNAVGEIVGIITRHNLTYEFLQARL RQHYQTI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256959 |
| ORF Size | 2541 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256959.1, NP_001243888.1 |
| RefSeq Size | 5647 bp |
| RefSeq ORF | 2544 bp |
| Locus ID | 1185 |
| UniProt ID | P51797 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Gene Summary | This gene encodes a member of the voltage-dependent chloride channel protein family. Members of this family can function as either chloride channels or antiporters. This protein is primarily localized to late endosomes and functions as a chloride/proton antiporter. Alternate splicing results in both coding and non-coding variants. Additional alternately spliced variants have been described but their full-length structure is unknown. [provided by RefSeq, Mar 2012] |
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