Tgfa (NM_012671) Rat Tagged ORF Clone

CAT#: RR204830

  • TrueORF®

Tgfa (Myc-DDK-tagged ORF) - Rat transforming growth factor alpha (Tgfa), (10 ug)



  "NM_012671" in other vectors (3)

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CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti TGF a (1-50) (rt); diluted antiserum
    • 500 Tests

CNY 11,025.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms RATTGFAA; TGFAA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR204830 representing NM_012671
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCCCGCGGCCGGACAGCTCGCTCTGCTAGCGCTGGGTATCCTGGTAGCTGTGTGTCAGGCTCTGG
AGAACAGCACGTCCCCCCTGAGTGACTCACCCGTGGCGGCTGCAGTGGTGTCTCACTTCAACAAGTGCCC
AGATTCCCACACTCAGTATTGTTTCCATGGGACCTGCAGGTTTTTGGTGCAGGAAGAGAAGCCAGCATGT
GTCTGCCACTCTGGGTACGTGGGTGTTCGCTGTGAGCATGCAGATCTCCTGGCAGTGGTGGCTGCCAGCC
AGAAGAAGCAGGCCATCACTGCCCTGGTGGTAGTCTCCATTGTGGCCCTGGCTGTCCTCATTATCACCTG
TGTGCTGATCCACTGCTGTCAGGTCCGCAAGCACTGTGAGTGGTGCCGTGCCCTCGTCTGCAGACACGAG
AAGCCCAGCGCCCTCCTGAAAGGAAGGACTGCCTGCTGCCACTCTGAGACAGTGGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR204830 representing NM_012671
Red=Cloning site Green=Tags(s)

MVPAAGQLALLALGILVAVCQALENSTSPLSDSPVAAAVVSHFNKCPDSHTQYCFHGTCRFLVQEEKPAC
VCHSGYVGVRCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQVRKHCEWCRALVCRHE
KPSALLKGRTACCHSETVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012671
ORF Size 477 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012671.2, NP_036803.1
RefSeq Size 4222 bp
RefSeq ORF 480 bp
Locus ID 24827
UniProt ID P01134
MW 17 kDa
Gene Summary acts as an EGF receptor ligand; inhibits apoptosis that results from epithelial detachment from the extracellular matrix [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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