Col4a1 (NM_001135009) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206114 representing NM_001135009 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCCGCTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC GGGCCGCTGCGAAGGGTGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGTGTGAAGGGACA AAAGGGAGAGAGAGGCTTTCCAGGGTTACAAGGTGTCATTGGATTTCCTGGGATGCAAGGACCTGAGGGG CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGAACGAAAGGGACACGAG GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAGGATGGTCCTCC AGGTCCCCCAGGTATCCCAGGATGCAATGGTACAAAGGGAGAGAGAGGGCCACTCGGTCCTCCTGGCTTG CCTGGATTCAGCGGGAATCCTGGACCACCGGGGTTACCAGGAATGAAGGGAGATCCCGGTGAAATTCTCG GCCATGTTCCTGGGACCCTGCTGAAAGGAGAGAGAGGATTTCCTGGTATCCCTGGGGCGCCGGGCTCACC AGGGTTGCCAGGACTCCAGGGACCTGTCGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT CCTGGACCTCCTGGAGAAAAGGGGCAGATGGGATCCAGCTTCCAAGGACCAAAAGGCGACAAGGGAGAAC AAGGGGTCAGTGGTCCCCCGGGAGTACCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTCCAGGGAACACCAGGATATGGAGAGAAAGGTGAACCT GGCAAGCCAGGACCTCGGGGAAAACCTGGAAAAGATGGTGAAAAAGGAGAAAAGGGGAGCCCGGGCTTTC CTGGTGAATCCGGATACCCAGGTCTTCCAGGCCGGCAGGGCCCTCAGGGAGAGAAGGGTGAACCTGGACT TCCAGGCCCCCCTGGAACTGTGGTAGGCACCAGGCCATTGGGAGAGAAAGGAGAGCGAGGCTACCCGGGA TCCCCAGGGTTAAGAGGAGAGCCCGGTCCTAAAGGTTACCCTGGATCGCCAGGCCAACCAGGCCCTCCAG GTTTCGCTGTTCCAGGCCAGACTGGTGCTCCGGGCTTCCCAGGTGAAAGAGGAGAAAAAGGTGAAAGGGG ATCTCCAGGTGTGTCTTTACCAGGACCAAGTGGAAGAGATGGAGCCCCAGGGCCTCCTGGCCCTCCTGGC CCCCCTGGGCAACCAGGCCACACGAACGGCATTGTGGAGTGTCAACCTGGACCACCTGGGGACCAGGGTC CTCCAGGAATTCCAGGACAGCCAGGTTTGACAGGCGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG CCTTGCCTGTGACACAGAAGGACTTCGAGGCCCCCCAGGGCCACAGGGGCCTCCAGGAGAGATTGGTTTC CCTGGACAGCCAGGGGCTAAAGGTGACCGAGGCCTGCCAGGCAGAGATGGTCTTGAAGGATTGCCGGGTC CACAAGGTTCACCGGGGCTCATAGGCCAGCCTGGAGCTAAGGGAGAGCCCGGCGAGATATTTTTTGACAT GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCAGGACAGCCAGGGATGCCAGGAAGAGCAGGAACT CCGGGAAGAGACGGCCACCCAGGACTCCCCGGACCCAAAGGCTCCCCGGGTTCAATAGGATTGAAGGGAG AGAGAGGTCCCCCTGGAGGAGTTGGCTTTCCTGGTAGTCGTGGAGACATTGGCCCTCCTGGACCCCCAGG AGTCGGTCCCATCGGTCCCATTGGTGAAAAAGGACAAGCAGGCCTTCCTGGGGGCCCCGGGTCCCCAGGT CTTCCAGGTCCAAAGGGTGAAGCAGGAAAGGTTGTCCCACTCCCCGGCCCCCCTGGAGCTGCAGGACTTC CAGGATCCCCTGGGTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGGCCAGGCAA CCCAGGAGAGAAGGGTGCTGTGGGCCAACCAGGAATTGGATTTCCCGGGCTTCCTGGCCCCAAAGGTGTT GATGGCTTGCCTGGAGAGATAGGACGACCGGGGAGTCCAGGTCGCCCTGGATTTAATGGCTTACCTGGCA ACCCAGGACCGCAAGGCCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAGCCAGGTCT TCCAGGTATCCCTGGCACACCCGGAGAGAAAGGCAGCATCGGGGGACCTGGCGTTCCAGGAGAACAGGGG TTGACTGGCCCTCCCGGACTCCAGGGGATCAGAGGTGACCCAGGACCTCCTGGGGTTCAAGGCCCTGCAG GTCCACCAGGGGTTCCAGGAATAGGACCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG TTCATCAGGTCCACCTGGAGTTAAAGGAGAGAAGGGATTCCCTGGATTCCCAGGACTGGATATGCCTGGC CCCAAAGGAGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGGCTCCCTGGCCTCCCTG GACAGCAGGGGACTCCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGCGTCATGGGAACCCC GGGACAACCAGGCTCACCAGGACCAGCAGGCGCCCCAGGGTTACCCGGAGAGAAAGGAGACCATGGCTTT CCGGGCTCCTCGGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGATGTCGGGCTTCCTGGCA TGCCAGGATCCATGGAGCACGTGGACATGGGCAGCATGAAGGGGCAGAAAGGAGACCAGGGAGAGAAAGG ACAAATTGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCCGGAACACCAGGAGTGCCCGGGAAGGAT GGGCAGGCAGGGCACCCTGGACAGCCAGGACCTAAAGGTGACCCAGGCCTTAGTGGAATACCAGGATCCC CTGGACTTCCTGGACCCAAAGGATCGGTTGGAGGAATGGGCTTGCCAGGTTCGCCCGGAGAGAAAGGCGT TCCGGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAAGGAGCTAAGGGAGAGAAAGGG CAGTCAGGTCTCCCTGGCATTGGAATTCCTGGACGGCCTGGTGACAAGGGAGATCAGGGGCTTGCAGGTT TCCCAGGCAGCCCTGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG CCCAAGAGGCTCTCCAGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAAAAAGGAGACAAA GGCCTCCCAGGACTGGATGGTGTTCCCGGTGTCAAAGGAGAAGCAGGTCTTCCTGGGACTCCTGGCCCCA CAGGCCCAGCTGGCCAGAAGGGAGAACCTGGCAGTGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA ACAAGGTTTGCCAGGAAGAGGCTTCCCAGGCTTCCCCGGCACCAAAGGAGACAAAGGTTCCAAGGGTGAA GTGGGTTTCCCTGGCCTTGCCGGAAGTCCTGGGATTCCTGGAGCCAAAGGCGAGCAAGGATTCATGGGTC CTCCCGGCCCTCAAGGACAGCCGGGCTTACCTGGCACTCCTGGCCACCCTGTGGAGGGGCCCAAAGGAGA CCGAGGACCTCAGGGTCAACCCGGCCTGCCAGGGCATCCGGGACCTATGGGGCCACCAGGGTTCCCTGGA ATCGATGGGCCAAAAGGTGACAAGGGAAATCCAGGTTGGCCAGGAGCTCCTGGGGCTCCAGGCCCTAAGG GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT GGGACCGCCTGGCGTTCCAGGATTCCAAGGTCAGAAAGGTCTTCCTGGCTTGCAGGGAGTGAAGGGAGAT CAGGGAGATCAAGGCATACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCCCCAGGTCCCTACGATG TCATCAAAGGAGAACCAGGGCTCCCAGGTCCTGAGGGTCCTCCTGGTCTCAAAGGACTTCAAGGACCACC AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCCGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTT GATGGTGCCCCTGGCCAGAAAGGAGAGACTGGACCATTTGGACCACCTGGTCCGAGAGGGTTTCCTGGTC CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCGGGTACCCCATCTGTGGACCATGGCTTCCT TGTGACCAGGCATAGTCAGACAACAGATGACCCGCTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGG TACTCTCTGCTCTATGTCCAAGGAAACGAGCGGGCCCATGGTCAGGACTTGGGTACGGCTGGCAGCTGTC TGCGCAAGTTCAGCACCATGCCCTTCCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA CGACTACTCCTACTGGCTGTCCACTCCGGAGCCCATGCCCATGTCCATGGCACCTATCTCTGGGGACAAC ATCCGGCCCTTCATTAGCAGGTGTGCAGTTTGCGAAGCGCCCGCCATGGTGATGGCGGTGCACAGTCAGA CCATCCAGATCCCGCAGTGCCCCAACGGTTGGTCCTCACTGTGGATTGGCTATTCCTTTGTGATGCACAC CAGCGCTGGTGCTGAAGGTTCTGGCCAAGCCCTTGCATCTCCCGGGTCCTGTCTGGAAGAGTTTAGAAGT GCCCCATTCATCGAGTGCCATGGCAGAGGAACGTGCAATTACTACGCGAATGCTTACAGCTTTTGGCTCG CCACCATAGAGAGAAGCGAGATGTTCAAGAAGCCCACGCCGTCCACCTTGAAGGCAGGGGAGCTGCGCAC GCATGTCAGCCGCTGCCAAGTGTGCATGAGAAGAACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206114 representing NM_001135009 Red=Cloning site Green=Tags(s) MGPRLSVWLLLPLAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGFPGLQGVIGFPGMQGPEG PHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL PGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGAPGSPGLPGLQGPVGPPGFTGPPGPPGP PGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFQGTPGYGEKGEP GKPGPRGKPGKDGEKGEKGSPGFPGESGYPGLPGRQGPQGEKGEPGLPGPPGTVVGTRPLGEKGERGYPG SPGLRGEPGPKGYPGSPGQPGPPGFAVPGQTGAPGFPGERGEKGERGSPGVSLPGPSGRDGAPGPPGPPG PPGQPGHTNGIVECQPGPPGDQGPPGIPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGF PGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGT PGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPIGEKGQAGLPGGPGSPG LPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGNPGEKGAVGQPGIGFPGLPGPKGV DGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQG LTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGVKGEKGFPGFPGLDMPG PKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGAPGLPGEKGDHGF PGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKD GQAGHPGQPGPKGDPGLSGIPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKG QSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDK GLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGLPGRGFPGFPGTKGDKGSKGE VGFPGLAGSPGIPGAKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPG IDGPKGDKGNPGWPGAPGAPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGQKGLPGLQGVKGD QGDQGIPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGF DGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHG YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDN IRPFISRCAVCEAPAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135009
ORF Size	5007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135009.1, NP_001128481.1
RefSeq Size	6579 bp
RefSeq ORF	5010 bp
Locus ID	290905
MW	160.6 kDa

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