Lrpap1 (NM_001169113) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216472 representing NM_001169113 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTCTTAGAGACAGGGTCTCTACGCTGCCCCGGCTCCAACTGCTAGTGCTGTTGTTGCTGCCGC TGCTGCTTGTGCCCCAGCCCATCGCAGGTCATGGCGGCAAGTACTCGCGGGAGAAGAATGAGCCCGAGAT GGCCGCCAAGCGTGAGTCCGGGGAGGAGTTCCGCATGGAGAAGCTGAACCAGCTATGGGAGAAGGCCAAG CGGCTGCATCTGTCTCCTGTGAGGCTGGCCGAGCTGCATTCTGACCTGAAGATACAAGAGAGGGATGAAC TCAACTGGAAGAAGCTGAAGGTGGAAGGCCTAGACGGGGATGGGGAGAAAGAAGCAAAACTGGTCCACAA TCTCAATGTCATCCTGGCCAGGTACGGACTGGATGGGAGGAAGGACACCCAGACAGTGCACAGCAACGCC CTCAATGAAGACACCCAGGATGAGCTGGGGGACCCCAGGTTGGAAAAGCTGTGGCACAAGGCAAAGACAT CAGGGAAATTCTCCAGTGAAGAACTGGACAAGCTGTGGCGAGAGTTTCTGCACTACAAAGAGAAGATCCA CGAGTACAATGTACTGCTAGATACACTGAGCAGAGCTGAAGAAGGTTATGAGAACCTTCTCAGCCCCTCT GACATGACCCACATCAAGAGTGACACCCTGGCCAGCAAGCACAGTGAACTGAAGGACAGACTGCGTAGTA TCAACCAGGGCCTAGACCGCCTGAGGAAGGTCAGCCACCAGGGCTACGGCCCCGCCACTGAGTTTGAAGA GCCCCGAGTGATAGATCTGTGGGACCTGGCTCAGTCTGCCAACTTCACCGAGAAGGAACTGGAGTCGTTC AGGGAGGAGCTTAAGCACTTCGAGGCCAAAATCGAAAAGCACAACCACTACCAGAAGCAGCTGGAGATTT CCCACCAGAAGCTGAAGCACGTGGAGAGCATCGGTGACCCCGAGCACATCAGCCGCAACAAGGAGAAGTA CGTCCTGCTGGAGGAGAAGACAAAGGAGCTGGGCTACAAGGTGAAGAAGCATCTGCAGGACCTGTCCAGC AGGGTCTCAAGGGCTCGGCACAATGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216472 representing NM_001169113 Red=Cloning site Green=Tags(s) MAPLRDRVSTLPRLQLLVLLLLPLLLVPQPIAGHGGKYSREKNEPEMAAKRESGEEFRMEKLNQLWEKAK RLHLSPVRLAELHSDLKIQERDELNWKKLKVEGLDGDGEKEAKLVHNLNVILARYGLDGRKDTQTVHSNA LNEDTQDELGDPRLEKLWHKAKTSGKFSSEELDKLWREFLHYKEKIHEYNVLLDTLSRAEEGYENLLSPS DMTHIKSDTLASKHSELKDRLRSINQGLDRLRKVSHQGYGPATEFEEPRVIDLWDLAQSANFTEKELESF REELKHFEAKIEKHNHYQKQLEISHQKLKHVESIGDPEHISRNKEKYVLLEEKTKELGYKVKKHLQDLSS RVSRARHNEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169113
ORF Size	1080 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169113.1, NP_001162584.1
RefSeq Size	1414 bp
RefSeq ORF	1083 bp
Locus ID	116565
UniProt ID	Q99068
MW	42 kDa
Gene Summary	Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.[UniProtKB/Swiss-Prot Function]

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