Sgk1 (NM_001193568) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sgk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216691 representing NM_001193568 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGATGCAGGGCGCGCTGGCTCGGGCTCGGCTCGAGTCCCTGCTCCGGCCCCGCCACAAAAAGC GAGTGGAGGCGCAGAAAAGGAGCGAGTCCGTCCTGCTAAGCGGACTGGCTTTCATGAAACAGAGAAGGAT GGGCCTGAACGATTTTATTCAGAAGCTTGCCAACAACTCCTATGCATGCAAACACCCTGAAGTTCAATCC TATTTGAAAATCTCCCAACCTCAGGAGCCCGAACTTATGAACGCCAACCCCTCACCTCCTCCAAGTCCCT CTCAACAAATCAACCTGGGTCCATCCTCAAATCCCCACGCCAAACCCTCTGACTTCCACTTCTTGAAAGT GATCGGAAAAGGCAGTTTTGGAAAGGTTCTTCTAGCAAGGCACAAGGCAGAAGAAGCATTCTATGCCGTC AAAGTTTTGCAGAAGAAAGCCATCTTGAAGAAGAAGGAGGAGAAGCATATTATGTCAGAGCGCAATGTTC TGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACAAACTCTA CTTCGTCCTAGACTACATTAATGGCGGAGAGCTGTTCTACCATCTCCAGAGGGAGCGCTGCTTCCTGGAA CCCCGTGCTCGCTTCTACGCAGCTGAAATAGCCAGTGCCTTGGGTTATCTGCACTCCCTAAACATCGTTT ATCGAGACTTAAAACCAGAGAATATTCTCCTAGACTCACAGGGACACATCGTCCTCACTGACTTTGGGCT CTGCAAGGAGAACATCGAGCACAATGGGACAACGTCCACCTTCTGTGGCACGCCTGAGTATCTCGCTCCT GAGGTTCTCCATAAGCAGCCGTACGACCGGACAGTGGACTGGTGGTGCCTCGGGGCTGTCTTGTATGAGA TGCTCTATGGCCTGCCTCCGTTCTACAGCCGGAACACAGCCGAGATGTATGACAATATTCTGAACAAGCC TCTCCAGCTGAAACCAAATATCACCAACTCAGCAAGGCACCTGCTGGAGGGCCTCCTGCAGAAGGACCGG ACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGAGTCATATTTTCTTCTCTTTGATTAACT GGGATGATCTCATTAATAAGAAGATCACGCCCCCATTTAACCCAAATGTGAGCGGGCCCAGTGACCTTCG GCACTTTGATCCCGAGTTTACTGAGGAGCCGGTCCCCAGCTCCATCGGGCGATCCCCTGACAGCATCCTT GTCACAGCCAGTGTGAAAGAAGCCGCGGAAGCCTTCCTTGGCTTCTCCTATGCCCCTCCTATGGACTCCT TCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216691 representing NM_001193568 Red=Cloning site Green=Tags(s) MGEMQGALARARLESLLRPRHKKRVEAQKRSESVLLSGLAFMKQRRMGLNDFIQKLANNSYACKHPEVQS YLKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEAFYAV KVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLE PRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNGTTSTFCGTPEYLAP EVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDR TKRLGAKDDFMEIKSHIFFSLINWDDLINKKITPPFNPNVSGPSDLRHFDPEFTEEPVPSSIGRSPDSIL VTASVKEAAEAFLGFSYAPPMDSFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001193568
ORF Size	1335 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001193568.1, NP_001180497.1
RefSeq Size	2555 bp
RefSeq ORF	1338 bp
Locus ID	29517
MW	50.6 kDa
Gene Summary	may play a role in menory consolidation and spatial learning; may mediate aldosterone-induced signaling [RGD, Feb 2006]

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