Il1rap (NM_001167840) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IL-1RAcp; Il1racpb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217103 representing NM_001167840 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTTCCTTGGTGTTTGATGAGTCTGTTCTTCTGTGGGATCCTGCAGAGTCACGCTTCCGAACGCT GTGATGACTGGGGACTAGATACCATGCGACAAATCCAAGTGTTTGAAGATGAGCCAGCTCGCATCAAGTG CCCCCTCTTTGAACACTTCTTGAAGTACAACTACAGCACTGCCCATTCCTCTGGACTTACCCTGATCTGG TACTGGACCAGGCAAGACCGGGACCTGGAGGAGCCCATTAATTTCCGCCTTCCAGAGAATCGCATCAGTA AGGAGAAAGATGTGCTCTGGTTCCGGCCCACCCTCCTCAATGACACGGGCAATTACACCTGCATGTTGAG GAACACGACTTACTGCAGCAAAGTTGCATTTCCCCTGGAAGTTGTTCAGAAGGACAGCTGTTTCAATTCC CCCATGAGACTCCCAGTGCACAGGCTGTATATTGAACAGGGTATTCACAATATCACGTGTCCAAATGTAG ATGGATATTTTCCTTCCAGTGTCAAACCATCGGTCACTTGGTATAAGGGTTGTACTGAAATAGTGAATTT TCATAATGTTCAACCCAAGGGCATGAACTTGAGTTTTTTCATCCCCTTGGTATCAAATAACGGAAATTAC ACATGTGTGGTGACGTATCTGGAAAACGGACGGCTCTTTCACCTCACCAGGACTATGACTGTAAAGGTGG TAGGCTCACCAAAGGATGCAGTACCACCTCATATCTACTCGCCAAATGACCGCGTTGTCTATGAGAAGGA ACCAGGAGAGGAGTTGGTTATTCCCTGTAAAGTCTATTTCAGTTTCATTATGGACTCTCACAATGAGATC TGGTGGACCATTGATGGAAAGAAGCCTGATGACGTCCCTGTTGACATCACTATTAATGAAAGCGTAAGTT ATTCTTCAACGGAAGATGAGACAAGGACTCAGATTTTGAGCATCAAGAAAGTCACCCCTGAGGATCTGAA GCGCAACTATGTCTGTCACGCTCGAAATGCCGAAGGGGAAGCCGAGCAAGCAGCCAAGGTGAAACAGAAA GTCATACCGCCAAGGTACACAGTAGAACTCGCCTGTGGTTTTGGAGCCACAGTCTTTCTGGTGGTGGTTC TCATCGTGGTTTACCACGTTTACTGGCTGGAGATGGTCCTCTTTTACCGGGCTCATTTTGGAACGGATGA AACAATTCTTGATGGAAAAGAATATGATATTTATGTTTCCTATGCAAGAAATGCAGAAGAAGAGGAGTTT GTGCTGCTGACACTACGTGGAGTTTTGGAGAATGAGTTCGGATACAAGCTGTGCATCTTCGACAGGGACA GCCTCCCTGGGGGAAATACGGTGGAAGCAGTGTTTGATTTCATTCAGCGGAGCCGAAGGATGATTGTTGT CCTGAGCCCAGACTATGTGACAGAAAAGAGCATCAGTATGCTGGAGTTTAAGCTGGGTGTCATGTGCCAG AACTCCATTGCCACTAAGCTCATTGTGGTGGAGTACCGTCCGCTTGAGCAACCCCACCCAGGCATTATGC AGCTGAAAGAGTCTGTGTCTTTCGTAAGCTGGAAGGGAGAAAAGTCCAAACATTCTGGCTCCAAGTTCTG GAAGGCCTTGCGCTTGGCTCTTCCCCTGAGAAGTCTGAGCGCCAGCTCCGGTTGGAATGAGAGCTGTTCT TCTCAGTCTGACATCAGTCTGGATCATGTTCAGAGGAGAAGTCGTTTGAAAGAGCCCCCAGAACTTCAAA GCTCAGAGAGGGTGTCTGGAGCAGCGCCAGCCCCGGGCACCATATCCAAGCACCGAGGCAAGCCCTCGGC AGCCTGTCGCTGCTGTGTCACCTACTGTGAAGGAGAAAGTCACCTCAGGAGCAAGAGCCGGGCAGAGATG CACACCCATCCCCAATGGGAAACACACCTCTGTAAGCCTGATCTCCAAGAGTCTGAAAGTCAGTGGATAC AAAATGGCACCAGACCCGAACCCGCTCCCCAGATCTCAGCTCTTGCACTCCGCCATTTTACAGATTTATC CAATAACAATGACTTTTATATTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217103 representing NM_001167840 Red=Cloning site Green=Tags(s) MGLPWCLMSLFFCGILQSHASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIW YWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNS PMRLPVHRLYIEQGIHNITCPNVDGYFPSSVKPSVTWYKGCTEIVNFHNVQPKGMNLSFFIPLVSNNGNY TCVVTYLENGRLFHLTRTMTVKVVGSPKDAVPPHIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEI WWTIDGKKPDDVPVDITINESVSYSSTEDETRTQILSIKKVTPEDLKRNYVCHARNAEGEAEQAAKVKQK VIPPRYTVELACGFGATVFLVVVLIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNAEEEEF VLLTLRGVLENEFGYKLCIFDRDSLPGGNTVEAVFDFIQRSRRMIVVLSPDYVTEKSISMLEFKLGVMCQ NSIATKLIVVEYRPLEQPHPGIMQLKESVSFVSWKGEKSKHSGSKFWKALRLALPLRSLSASSGWNESCS SQSDISLDHVQRRSRLKEPPELQSSERVSGAAPAPGTISKHRGKPSAACRCCVTYCEGESHLRSKSRAEM HTHPQWETHLCKPDLQESESQWIQNGTRPEPAPQISALALRHFTDLSNNNDFYIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001167840
ORF Size	2055 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001167840.1, NP_001161312.1
RefSeq Size	2852 bp
RefSeq ORF	2058 bp
Locus ID	25466
MW	78.4 kDa
Gene Summary	facilitates the binding between interleukin-1 and its receptor [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.