DNMT1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	DNMT1
Locus ID	1786
Kit Components	KN217851G1, DNMT1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGGGCTGGGGCGGTACGCGC KN217851G2, DNMT1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TACCGCCTGCGGACATCGTC KN217851-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAAAAGGCAA CAAAAAACCC CCCACACACT GGGTATAGAA GTGGCATGGG CCTCTATACA CTGTGAGATT CTTGGTACTA GCTACAAATT CTGTGTATAC TCAAGATTTT CTAGAGTAGG TGCAATTACC CCGTTTTACA GATGAGGACA CAGAGGCTGA GCCGTAGTGA CCCACCTAAG GTCGTATAGC CAGCAAATAG ATGGAGGTTG GATTGGAACT GAGGACTTTA CTCAAGGGCT CTCACAAACC CTTGGGGGCT TCTCGCTGCT TTATCCCCAT CACACCTGAA AGAATGAATG AATGAATGCC TCGGGCACCG TGCCCACCTC CCAGCAAACC GTGGAGCTTG GACGAGCCCA CTGCTCCGCG TGGGGGGGGT GTGTGCCCGC CTTGCGCATG CGTGTTCCCT GGGCATGGCC GGCTCCGTTC CATCCTTCTG CACAGGGTAT CGCCTCTCTC CGTTTGGTAC ATCCCCTCCT CCCCCACGCC CGGACTGGGG TGGTAGACGC CGCCTCCGCT CATCGCCCCT CCCCATCGGT TTCCGCGCGA AAAGCCGGGG CGCCTGCGCT GCCGCCGCCG CGTCTGCTGA AGCCTCCGAG AACACGGACT CAGGGGGACA GGCAGGGCGC TGGGTGGGGG GTCGCTTCCC CTCGGGGTGG CCGGTGGCCG CTGCTGACAG ACGGGCGCGC ATGCGTGGGG TGGTGCGGCG CGCAGCGGCA GTTGGCGCGG GCAGGGTGGC ACTTCCGGTC GCGCGTGCCC GGGCTGTTTG GCGCCAAAAT GGACCGTGGA TTCCCCCGTA GCTCCCTGGT GGCTAGAAAC TAGGCGGGGT GGGGGTTCTC TTTTGATCCC CAAATACAGC AAGCTTTGGG TTCGTTTCCG GGGTCCCCTT CTTCAAGCAG CGGTGGGCCG GGCTCTTGAC TCCAGCTTGG TCGCACAGGA AGTGGGCAGC CCGCGGCCAA CGGACACCCC TCGGGCACCG GCACTTCGGC CTCCACTTCC GGTGTCCGGC CCGGTCCCCG GGGGCGCTTC TGTGGTGGGG GGTCCCTCAG TGCCTTTCCC CCAAAGCTGT GCATCTCGAT GGCGGCCTCC AGAGACGGAT GTCACCCGTC GGGGCCTGGG CTGGGCTGGC TCATGTTCGA GAGCCCAGCC TTCCGTGGGG TACTCATTCT GTCAGTTGGG ACTGTGTGGT TCTTGCTCCT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001130823, NM_001318730, NM_001318731, NM_001379
Synonyms	ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Summary	This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

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