Streptavidin Protein

Specifications

Product Data
Description	Streptavidin protein, 5 mg
Protein Source	S. avidinii
Predicted MW	53 kDa
Purity	>95% pure (SDS-PAGE)
Buffer	Presentation State: Purified State: Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free) Buffer System: PBS Preservative: 1% Mannitol Stabilizer: 1% Dextran
Bioactivity	Biological: One unit will bind 1 µg of D-Biotin. Specific: 17 U/mg Streptavidin
Reconstitution	Restore with distilled water or saline.  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Preparation	Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free)
Applications	Can be used for biotinylated antibody detection and quantification by ELISA, blotting and immunohistology; as a ligand for immobilization on activated matrices.
Protein Description	Purified Streptavidin prepared by Affinity Chromatography
Note	pI: 5
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
Summary	Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.