HSPA5 / GRP78 (Active Protein) Human Protein
CAT#: AR03021PU-L
HSPA5 / GRP78 (Active Protein) human recombinant protein, 2x0.1 mg
Size: 50 ug
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CNY 9,340.00
货期*
详询
规格
Cited in 7 publications. |
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Concentration | lot specific |
Purity | >90% pure as determined by SDS-PAGE analysis |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 50 mM Tris/HCl, pH 7.5, 150 mM NaCl and 10% Glycerol |
Preparation | Liquid purified protein |
Applications | Western Blot Control. ATPase activity. This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay. Binding Assay. ELISA reference standard. |
Protein Description | Recombinant Human GRP78 Protein with ATPase activity, His-tagged. |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_005338 |
Locus ID | 3309 |
UniProt ID | P11021, V9HWB4 |
Cytogenetics | 9q33.3 |
Synonyms | BIP; GRP78; HEL-S-89n |
Summary | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. [provided by RefSeq, Jul 2020] |
Protein Families | Druggable Genome |
Protein Pathways | Antigen processing and presentation, Prion diseases |
Citations (7)
The use of this Proteins has been cited in the following citations: |
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ER stress sensor, glucose regulatory protein 78 (GRP78) regulates redox status in pancreatic cancer thereby maintaining “stemness”
,null,
Cell Death & Disease
,PubMed ID 30755605
[HSPA5]
|
HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer
,null,
Autophagy
,PubMed ID 29260979
[HSPA5]
|
Glucose-regulated protein 78 autoantibody associates with blood-brain barrier disruption in neuromyelitis optica
,null,
Science translational medicine
,PubMed ID 28679661
[HSPA5]
|
Human GRP78 affinity towards its signaling partners Ire1α and PERK is differently modulated by an unfolded protein client.
,null,
Biochemical and biophysical research communications
,PubMed ID 28416388
[HSPA5]
|
Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella.
,null,
Cellular microbiology
,PubMed ID 27185209
[HSPA5]
|
Discovery of a Novel Target for the Dysglycemic Chromogranin A Fragment Pancreastatin: Interaction with the Chaperone GRP78 to Influence Metabolism
,null,
PLoS ONE
,PubMed ID 24465394
[HSPA5]
|
Glucose-Regulated Protein 78 Controls Cross-talk between Apoptosis and Autophagy to Determine Antiestrogen Responsiveness
,null,
Cancer research
,PubMed ID 22752300
[HSPA5]
|
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