Galectin-8 (1-317, His-tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW
Tag	His-tag
Predicted MW	37.9 kDa
Concentration	lot specific
Purity	>90% by SDS - PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl, 1 mM DTT, 10% glycerol
Preparation	Liquid purified protein
Applications	Protocol: Biological Assay : 1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g, Sodium citric aci d 0.8g, Citric acid 0.055 g, D - glucose 2.05g in DW100 ml) 2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS. 3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension. 4. Incubate for 1h at 37°C and wash four times with PBS. 5 . Dilute packed cells in PBS to give 4% red cell suspension. 6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4%  Red Cell - in - PBS in u shaped wells. 7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate) 8 . Incubate for 30min at room temperature to observe visible agglutination.
Protein Description	Recombinant human LGALS8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: The ED50 for this effect is is 5-10 ug/ml,  measured by its ability to agglutinate human red blood cells. (Biological assay see "Protocols").
Reference Data
RefSeq	NP_006490
Locus ID	3964
UniProt ID	O00214
Cytogenetics	1q43
Synonyms	Gal-8; PCTA-1; PCTA1; Po66-CBP
Summary	This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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