FGF basic / FGF2 Rat Protein

Specifications

Product Data
Species	Rat
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) ALPEDGGGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKGPG QKAILFLPMS AKS Result by N-terminal sequencing: ALPEDGGGAF PP
Predicted MW	16.34 kDa
Purity	>98% pure by SDS-PAGE
Buffer	Presentation State: Purified State: Lyophilized freeze dried protein without additives. Buffer System: 0.5x PBS without stabilizers
Preparation	Lyophilized freeze dried protein without additives.
Protein Description	Recombinant Rat Fibroblast Growth Factor-2. The cDNA of native rat FGF-2 (Ala11-Ser154) was cloned from total RNA derived from a rat embryo using standard protocols.
Storage	The lyophilized Rat FGF-2, though stable at room temperature, is best stored in working aliquots at –20°C to –70°C. Avoid repeated freeze-thaw cycles!
Bioactivity	Biological: The activity was determined by the stimulation of HUVE cells. The ED50 for this effect is ≤ 0.1ng/ml corresponding to a specific activity of ≥ 1 x 10e7 units/mg.
Endotoxin	< 0.1ng per μg of Rat FGF-2
Reference Data
RefSeq	NP_062178
Locus ID	54250
UniProt ID	P13109
Cytogenetics	2q25
Synonyms	bFGF; Fgf-2
Summary	activates the MAP kinase signaling pathway; plays a role in synaptic transmission; induces cell proliferation [RGD, Feb 2006]

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