CKMB Type 2 Protein

Specifications

Product Data
Expression Host	Pichia pastoris
Concentration	lot specific
Purity	>95% pure by SDS-PAGE. Purified under non-denaturing conditions.
Buffer	Presentation State: Azide Free State: Liquid purified protein Buffer System: 20 mM Tris-HCl, 1 mM DTT, 1 mM EDTA, pH 6.6-7.0 containing 50% (v/v) Glycerol Preservative: None
Preparation	Liquid purified protein
Applications	Suitable for use in ELISA.
Protein Description	Recombinant Human Creatine Kinase MB (CK-MB) Isoenzyme II expressed in Pichia pastoris. Recombinant full length Creatine Kinase MB Isoenzyme with amino acid sequence identical to native enzyme. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 47 kDa is not resolved on most gels. Purified in the enzymatically active form. Reacts with monoclonal antibodies specific to MB isoenzyme in ELISA.  Enzyme Activity: Kinetic Assay, ΔAbs measured at 340 nm. Acceptable Range: > 700 U/mg measured at 37°C.  Testing Results: 1097 U/mg.
Storage	Upon receipt, store (in aliquots) at -20°C to -80°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data

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