PDGFA (PDGF-AB) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	25.5 kDa
Purity	> 95% by SDS-PAGE and visualised by silver stain.
Buffer	50 mM Acetic Acid
Preparation	Lyophilized purified fraction.
Protein Description	Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
Note	Always centrifuge product before opening vial!
Storage	Store The lyophilized PDGF-AB for one month at room temperature or -20°C. Reconstituted PDGF-AB should be stored in working aliquots at -20°C to -70°C. Avoid repeated freeze-thaw cycles!
Stability	Shelf life: One year from despatch.
Bioactivity	Biological: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml. Specific: > 1 x 10e6 units/mg.
Endotoxin	< 0.1 ng per ug of PDGF-AB.
Reference Data
RefSeq	NP_002598
Locus ID	5154
UniProt ID	P04085
Cytogenetics	7p22.3
Synonyms	PDGF-A; PDGF1
Summary	This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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