PGK1 (NM_000291) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Ser2-Ile417
Tag	C-His
Predicted MW	45.5 kDa
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Provided lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl
Storage	Store at -80°C.
Stability	Stable for at least 3 months from date of receipt under proper storage and handling conditions.
Endotoxin	Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
Reference Data
RefSeq	NP_000282
Locus ID	5230
UniProt ID	P00558, V9HWF4
Refseq Size	2439
Cytogenetics	Xq21.1
Refseq ORF	1251
Synonyms	HEL-S-68p; MIG10; PGKA
Summary	The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]
Protein Families	Druggable Genome
Protein Pathways	Glycolysis / Gluconeogenesis, Metabolic pathways

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