pLenti-EF1a-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

CAT#: PS100121

pLenti-EF1a-C-mGFP-P2A-Puro, Lenti vector with EF1a promoter, C-terminal mGFP tag and P2A-Puro, 10ug



CNY 7,130.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

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TurboFectin Transfection Reagent (1 mL in 1 vial)
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Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

CNY 5,420.00

Specifications

Product Data
C-Tag mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Cell Selection Puromycin
Features

This Lenti vector is a third generation lentiviral vector.

  • The ORF cloned in this vector can be expressed as a tagged protein with C-terminal mGFP tag.
  • The expression of the cloned ORF is driven by EF1a promoter, a constitutive promoter.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34ug/ml)
  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • mGFP_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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