Secondary Antibodies

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, Aff - Purified

Applications Immunocytochemistry. 
Western Blotting: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Flow cytometry: 1-4 μg/ml.
ELISA: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, Purified

Applications Flow Cytometry: 20 µg/ml.
Immunohistochemistry on frozen sections: 20 µg/ml.
ELISA: 5 µg/ml as coating Antibody.
Immunoprecipitation.
Reactivities Human
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, APC

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation APC

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Applications FLISA: 1/200-1/400. 
Flow Cytometry: ≤ 1 µg/106 cells.
Identification and enumeration of IgG2a+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). 
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation PE

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
 ≤ 1 µg/106 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Azide Free

Applications ELISA.
FLISA: 1 µg/ml. 
Western blotting.
Dot- and Slot-Immunoblotting
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated