Secondary Antibodies

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified

Applications ELISA: 1/4000-1/8000 (See also Ref.2-9).
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation HRP

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified

Applications ELISA: 1/1000-1/2000 (See also Ref.2-9).
FLISA.
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation AP

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
General Recommended Dilutions:
Immunocytochemistry.
Immunohistochemistry (Paraffin).
Histochemical Use: 1/10-1/40.
ELISA: 1/100-1/2000.
Western blot: 1/200-1/4000.
Reactivities Human
Conjugation Biotin

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry:
reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

mIgG (1,2a,2b,3) Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9F11

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse, Rat
Conjugation Unconjugated

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hIgG1 and hIgG2 and hIgG3 F(ab')2 Fragment Specific Rabbit monoclonal antibody,OTIR4H6

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Human
Conjugation Unconjugated

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hIgG Kappa light chain Specific Rabbit monoclonal antibody, OTIR1G7

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Human
Conjugation Unconjugated

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mIgG2a Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR6C3

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

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mIgG1 Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR8A7

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

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mIgG3 Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR8B8

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

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mIgG2a Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9D3

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

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mIgG2a and mIgG2b Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9H1

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

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