Secondary Antibodies

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), TRITC

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA, and Immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation TRITC

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), FITC

Applications To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone NI 307, HRP

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone NI 307, Biotin

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Conjugation Biotin

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), HRP

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgA2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), HRP

Applications To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/50 upwards
Western blot: from 1/100 upwards
Reactivities Human
Conjugation HRP

Kappa light chain, mouse anti human, clone κ -117 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Lambda light chain, mouse anti human, clone λ-1 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Biotin

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1/10.
Reactivities Chicken, Duck
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin