Rabbit Monoclonal Anti-Human Lambda Light Chain antibody, clone RM127
Applications | ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL |
Reactivities | Human lambda light chain |
Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human Lambda Light Chain antibody, clone RM127
Applications | ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL |
Reactivities | Human lambda light chain |
Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Mouse IgM (mu-Chain) antibody, clone RM109
Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL |
Reactivities | Mouse IgM mu-chain |
Conjugation | Unconjugated |
Goat Monoclonal Anti-Rabbit IgG Fc antibody, clone RMG02
Applications | ELISA: 0.01ug/mL – 0.5ug/mL, ICC/IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL |
Reactivities | Rabbit IgG Fc |
Conjugation | Unconjugated |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Azide Free
Applications | ELISA. FLISA: 1 µg/ml. Western blotting. Dot- and Slot-Immunoblotting Immunocytochemistry. Immunohistochemistry on Frozen Sections. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified
Applications | ELISA: 1/1000-1/10000. Immunofluorescence. |
Reactivities | Human |
Conjugation | FITC |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified
Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1-10 µg. |
Reactivities | Chicken |
Conjugation | Unconjugated |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
Reactivities | Human |
Conjugation | Unconjugated |
Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE
Applications | Flow Cytometry: < / = 0.2 µg/10e6 cells. Identification and enumeration of IgE+ cells. Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells. Also used as a second step reagent for mouse IgM monoclonal antibodies. |
Reactivities | Mouse |
Conjugation | PE |
Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE
Applications | Flow Cytometry: < / = 0.2 µg/10e6 cells. Identification and enumeration of IgE+ cells. Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells. Also used as a second step reagent for mouse IgM monoclonal antibodies. |
Reactivities | Mouse |
Conjugation | PE |
Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, FITC
Applications | Flow Cytometry: < / = 1 µg/10e6 cells. (See Ref.1-5) Immunofluorescence. |
Reactivities | Mouse |
Conjugation | FITC |
Human IgG mouse monoclonal antibody, clone 4D2D9G8, Purified
Applications | Western Blot: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. |
Reactivities | Human |
Conjugation | Unconjugated |