Secondary Antibodies

Human IgE mouse monoclonal antibody, clone NI 307, Biotin

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Conjugation Biotin

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Biotin

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), Biotin

Applications To identify the presence of IgA1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/250 upwards. 
Western blot: 1/500 upwards.
Reactivities Human
Conjugation Biotin

Kappa light chain, mouse anti human, clone κ -117 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Lambda light chain, mouse anti human, clone λ-1 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation Biotin

Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin

Applications Flow Cytometry (< / = 1.0 µg/10e6 cells).
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Biotin

Rabbit IgG mouse monoclonal antibody, clone 2A9, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129

Applications ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human Ig light chain
Conjugation Biotin