Secondary Antibodies

Rabbit Monoclonal Anti-Human IgG3 antibody, clone RM119

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgG3
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgG2b (gamma-2b) antibody, clone RM108

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (stronger on non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG2b
Conjugation Unconjugated

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine
Conjugation Unconjugated

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, Purified

Applications Flow Cytometry: 20 µg/ml.
Immunohistochemistry on frozen sections: 20 µg/ml.
ELISA: 5 µg/ml as coating Antibody.
Immunoprecipitation.
Reactivities Human
Conjugation Unconjugated

Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites

Applications Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting.
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards.
Western blotting: 1/2000-1/10000.
Reactivities Human
Conjugation Unconjugated

Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites

Applications Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards.
Western blotting: 1/2000 Upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Purified

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG4, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Ascites

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Purified

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated