Secondary Antibodies

Rat IgG2b (subclass specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended working dilutions:
Histochemistry: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Rat
Conjugation Unconjugated

Rat IgE (Fc specific) goat polyclonal antibody, TRITC

Applications This antibody can be used:
To identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence
staining of appropriately treated cell and tissue substrates.
To identify circulating IgE antibodies in Rat serum or other body fluids.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions: 1/10-1/60.
Reactivities Rat
Conjugation TRITC

Rat IgE (Fc specific) goat polyclonal antibody, FITC

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To identify and measure IgE in Rat serum or other body fluids.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions: 1/20-1/80.
Reactivities Rat
Conjugation FITC

Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Applications IF: 1:100 - 1:250
FC: 1:100 - 1:250
Reactivities Rat
Conjugation Phycoerythrin

Rat IgG2a goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat
Conjugation Unconjugated

Rat IgG2b rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgG1 goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgM goat polyclonal antibody, Purified

Applications ELISA 1ug/ml - 10ug/ml, Western Blotting, Flow Cytometry
Reactivities Rat
Conjugation Unconjugated

Rat IgA goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Immunofluorescence.
Reactivities Reptiles, Rat
Conjugation Unconjugated

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Purified

Applications ELISA.
Flow Cytometry:
5 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000. 
Western Blot. 
Immunofluorescence. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections.

Reactivities Rat
Conjugation Biotin

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Reactivities Rat
Conjugation HRP