Recombinant Proteins

Proteins

Recombinant Proteins

Human

IRF5

MORTALITY FACTOR 4 LIKE 2

TNF ALPHA

HMGA1

PQBP1

MAX

HMG1

HNF 4 ALPHA

CYCLOPHILIN E

ESTROGEN RELATED RECEPTOR GAMMA

HNRNPD

IKK GAMMA

P53

PPAR GAMMA

RNF14

SUPT5H

APE1

BETA CATENIN

HIP2

SOD2

EDF1

ESTROGEN RECEPTOR 1

GROWTH ARREST SPECIFIC PROTEIN 7

NFYA

NRG1

PAX8

PROTEIN KINASE A REGULATORY SUBUNIT I ALPHA

SIGIRR

STAT1

STAT3

TGF BETA 1

BMAL1

CITED1

DAXX

HOPX

KAT5

MEIS2

NACA

NFAT1

NONO

NUCLEOPHOSMIN

PML PROTEIN

TBL1Y

TCF4

TGIF

VGL4

ZNF580

B7-1

EED

HMGB2

EIF4EBP2

GMEB1

HSF2

MIZ1

NCOA4

PCDH1

PEROXIREDOXIN 3

RBPJK

SATB1

STAT5B

STAT6

TCF3 / E2A

TFIIB

ZNF205

AMINO TERMINAL ENHANCER OF SPLIT

BACH1

C-MYC

CAMKK2

CELF3

CERAMIDE SYNTHASE 2

CNBP

CSEN

CTNNBIP1

DYNAMIN 2

ELOB

ERG

FE65

FOXO3

FOXP4

GATA3

GRAINYHEAD LIKE PROTEIN 1 HOMOLOG

GTF2IRD1

GTF3C2

HAIRLESS

HIC5

HIF1 BETA

HNF1 ALPHA

IKB BETA

ING4

IRF3

KEAP1

MCM4

MEK3

MLX

MSK1

NFKB P100 / P52

NFYC

NIPP1

NR2E3

NUCLEAR FACTOR 1

HEK293T

E. coli

Sf9

HEK293

CHO

Insect

C-Myc/DDK

N-His

N-GST and C-His

C-His

Tag Free

C-DDK

N-GST and C-HIS

N-GST

His-tag

Myc-DDK

N-His&SUMO

N-His-ABP (Albumin-Binding Protein)

N-MARI

N-6His

C-DDK/His

N-GST, C-His

N-His-GST

C-6×His

C-Fc

C-Fc/His

C-Mouse Fc and 6×His

C-mFc

N-6His-FLAG

N-Avi

N-His&Flag

N-His/DDK

Tag-free

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

ELISA

Stem cell relevant signaling - Wnt Signaling pathway

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Pathways in cancer

MAPK signaling pathway

Toll-like receptor signaling pathway

Wnt signaling pathway

Cell cycle

TGF-beta signaling pathway

Chronic myeloid leukemia

Pancreatic cancer

Metabolic pathways

T cell receptor signaling pathway

Adipocytokine signaling pathway

Acute myeloid leukemia

Chemokine signaling pathway

Huntington's disease

Small cell lung cancer

Apoptosis

Neurotrophin signaling pathway

Prostate cancer

Thyroid cancer

RIG-I-like receptor signaling pathway

Purine metabolism

Pyrimidine metabolism

Cytosolic DNA-sensing pathway

Jak-STAT signaling pathway

Ubiquitin mediated proteolysis

Colorectal cancer

RNA polymerase

B cell receptor signaling pathway

NOD-like receptor signaling pathway

Amyotrophic lateral sclerosis (ALS)

Basal transcription factors

Maturity onset diabetes of the young

Natural killer cell mediated cytotoxicity

Renal cell carcinoma

Systemic lupus erythematosus

Allograft rejection

Graft-versus-host disease

Type I diabetes mellitus

Spliceosome

Endometrial cancer

ErbB signaling pathway

Adherens junction

Antigen processing and presentation

Cytokine-cytokine receptor interaction

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

Base excision repair

Fc epsilon RI signaling pathway

Basal cell carcinoma

Insulin signaling pathway

Type II diabetes mellitus

Alzheimer's disease

Bladder cancer

Non-small cell lung cancer

Endocytosis

PPAR signaling pathway

Tight junction

p53 signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Nucleotide excision repair

Asthma

Hematopoietic cell lineage

Melanoma

Notch signaling pathway

RNA degradation

Focal adhesion

Axon guidance

Primary immunodeficiency

Oocyte meiosis

VEGF signaling pathway

Glioma

Melanogenesis

Viral myocarditis

Autoimmune thyroid disease

Cell adhesion molecules (CAMs)

DNA replication

Pathogenic Escherichia coli infection

Fc gamma R-mediated phagocytosis

Leukocyte transendothelial migration

Circadian rhythm - mammal

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Regulation of actin cytoskeleton

Dorso-ventral axis formation

Cysteine and methionine metabolism

GnRH signaling pathway

Neuroactive ligand-receptor interaction

Hedgehog signaling pathway

Long-term depression

Valine

Glycolysis / Gluconeogenesis

Lysine degradation

Ribosome

Aminoacyl-tRNA biosynthesis

Glycerolipid metabolism

Glycine

Glyoxylate and dicarboxylate metabolism

Homologous recombination

Long-term potentiation

Nicotinate and nicotinamide metabolism

Non-homologous end-joining

Progesterone-mediated oocyte maturation

Proteasome

leucine and isoleucine biosynthesis

leucine and isoleucine degradation

mTOR signaling pathway

serine and threonine metabolism

Vascular smooth muscle contraction

Human

E. coli

Human 293

1

2

Yes

In Stock